Objective:To investigate the protective effect of diallyl sulfide and the therapeutic window of opportunity for diallyl sulfide following focal cerebral ischemia/reperfusion injury in rats.Methods:For the protective effect of diallyl sulfide

focal cerebral ischemia-/reperfusion models in rats were induced by the transient occlusion of the middle cerebral artery(MCAO)for 2 h

followed by reperfusion for 24 h

and the animals were administered DAS25

50

100

200 mg/kg

ip

q d × 7 d before transient MCAO.The neurological deficit score

infarct size and brain edema were examined.For the therapeutic window of opportunity

the MCAO model was induced by transient occlusion for 2 h and reperfusion for 24 h.The drugs were administered at 1h after the onset of ischemia and 2

4

6 h after reperfusion respectively.The neurological deficit score

infarct size and brain edema were examined.Results:DAS 50 mg/kg

ip

q d × 7 d before MCAO significantly reduced the neurological deficit score

infarct size and brain edema.The administration of DAS beginning at 1 h after the onset of ischemia significantly reduced neurological deficit sore

infarct size and brain ischemia;starting at 2

4 h after reperfusion

the neuroprotective effects decreased

and starting at 6 h after reperfusion did not result in significant reduction of the above indexes for brain tissue.Conclusion:Diallyl sulfide has protective effect on cerebral ischemia/reperfusion injury.The therapeutic window of opportunity of diallyl sulfide in transient focal cerebral ischemia injury is between 1 h after the onset of ischemia and 2 h after reperfusion.