shRNA干扰PARG对大肠癌CT26细胞粘附因子表达的影响

颜佳欣; 王娅兰; 吴伟强; 潘娟

doi:10.13406/j.cnki.cyxb.2011.06.029

您当前的位置：

首页 >

文章列表页 >

shRNA干扰PARG对大肠癌CT26细胞粘附因子表达的影响

恶性肿瘤的分子诊断 | 更新时间：2024-01-17

- shRNA干扰PARG对大肠癌CT26细胞粘附因子表达的影响
- Influence of shRNA interfering with PARG on the expression of cell adhesion fact
- 重庆医科大学学报 2011年36卷第6期页码：641-645
- 作者机构：
  
  重庆医科大学基础医学院病理学教研室分子医学与肿瘤研究中心
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目（编号:NSFC30870946）
- DOI：10.13406/j.cnki.cyxb.2011.06.029
  中图分类号： R735.34
- 纸质出版日期：2011，
扫描看全文
[1]颜佳欣,王娅兰,吴伟强,潘娟.shRNA干扰PARG对大肠癌CT26细胞粘附因子表达的影响[J].重庆医科大学学报,2011,36(06):641-645.

YAN Jia-xin, WANG Ya-lan, WU Wei-qiang, et al. Influence of shRNA interfering with PARG on the expression of cell adhesion fact[J]. Journal of Chongqing Medical University, 2011,36(6):641-645.
[1]颜佳欣,王娅兰,吴伟强,潘娟.shRNA干扰PARG对大肠癌CT26细胞粘附因子表达的影响[J].重庆医科大学学报,2011,36(06):641-645. DOI： 10.13406/j.cnki.cyxb.2011.06.029.

YAN Jia-xin, WANG Ya-lan, WU Wei-qiang, et al. Influence of shRNA interfering with PARG on the expression of cell adhesion fact[J]. Journal of Chongqing Medical University, 2011,36(6):641-645. DOI： 10.13406/j.cnki.cyxb.2011.06.029.

摘要

目的:探讨小鼠大肠癌CT26株中聚（腺苷二磷酸核糖）水解酶[Poly（ADP-ribose）glycohydrolase

PARG]对细胞间粘附因子表达的影响及其可能的机制。方法:以慢病毒为载体对CT26细胞株中PARG基因进行RNA沉默干扰

并筛选出稳定表达株。Realtime-PCR法检测沉默效果。Western blot法检测CT26细胞PARG、PARP、NF-κB和Pi-AKT（473）I、CAM-1、P-selectin的蛋白表达。结果:实验组与对照组（包括阴性对照组、空载体对照组）相比

PARG、PARP、Pi-AKT（473）、NF-κBI、CAM-1、P-selectin表达经统计学分析差异均有统计学意义（P<0.05）。结论:在大肠癌细胞株中

抑制PARG可以通过调节与NF-κB有关通路

进一步调节ICAM-1、P-selectin等细胞粘附因子。提示PARG在肿瘤癌栓形成过程中可能具有重要作用。

Abstract

Objective:To clarify the possible mechanism of how PARG and PARP are to regulate intercellular adhesion factor through relevant signal pathways.Methods:Lentivirus carried shRNA interfered with the expression of PARG and steadily silenced cell lines.Realtime-PCR was used for testing the strength of shRNAi and Western blot was used to test the expressions of PARG

PARP

NF-κB

and Pi-AKT(473)

and LY249002 was served as Pi-AKT inhibitor.Results:The expressions of PARG

PARP

and NF-κB

ICAM-1

P-selectin in shRNAi group were weaker than those in the control groups(blank control and transfected with empty vector control group);whereas Pi-AKT increased in the shRNAi group(P<0.05).Conclusion:The results suggest that PARG gene silencing may regulate NF-κB related signaling pathway and the intercellular adhesion factors such as ICAM-1 and P-selectin

which are reduced.

关键词

PARG慢病毒转染AKT磷酸化细胞粘附

Keywords

poly(ADP-ribose)glycohydrolaselentiviral transfectionAKT phosphorylationcell adhesion

references

浏览量

112

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

卡介菌多糖核酸对肺癌细胞与脐静脉血管内皮细胞粘附力学特性影响

抗CXCR4单克隆抗体12G5对胃癌细胞粘附与侵袭的影响

大肠癌细胞PARG、PARP与AKT磷酸化状态的关系

shRNA claudin-1对SW620细胞侵袭转移的影响