脉络膜新生血管CNV动物模型的评估新方法

许琴; Nalini S.Bora; Puran Bora

doi:10.13406/j.cnki.cyxb.2011.02.037

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脉络膜新生血管CNV动物模型的评估新方法

技术与方法 | 更新时间：2024-01-17

- 脉络膜新生血管CNV动物模型的评估新方法
- A new assessment method for animal model of choroidal neovascularization (CNV)
- 重庆医科大学学报 2011年36卷第2期页码：192-194
- 作者机构：
  
  1. 贵州遵义医学院附院检验科
  2. University of Arkansas
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.02.037
  中图分类号： R774.5
- 纸质出版日期：2011，
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[1]许琴,Nalini S.Bora,Puran Bora.脉络膜新生血管CNV动物模型的评估新方法[J].重庆医科大学学报,2011,36(02):192-194.

XU Qin, et al. A new assessment method for animal model of choroidal neovascularization (CNV)[J]. Journal of Chongqing Medical University, 2011,36(2):192-194.
[1]许琴,Nalini S.Bora,Puran Bora.脉络膜新生血管CNV动物模型的评估新方法[J].重庆医科大学学报,2011,36(02):192-194. DOI： 10.13406/j.cnki.cyxb.2011.02.037.

XU Qin, et al. A new assessment method for animal model of choroidal neovascularization (CNV)[J]. Journal of Chongqing Medical University, 2011,36(2):192-194. DOI： 10.13406/j.cnki.cyxb.2011.02.037.

摘要

目的:建立C57BL/6小鼠脉络膜新生血管（Choroidal neovascularization

CNV）动物模型的评估方法。方法:①应用氪红激光对小鼠实验性视网膜进行光凝

激光的功率、光凝斑直径和曝光时间分别为250 mW、50μm及0.05 s诱导CNV动物模型。②分别于光凝后1、3、5、7、10d

用2×106FITC-dextran荧光素灌注小鼠心脏。③mAb特定抗Elastin抗体标记Bruch膜。④视网膜色素上皮（Retinal pigmentepithelium

RPE）-脉络膜-巩膜复合体铺片。⑤激光共聚焦显微镜对RPE-脉络膜-巩膜复合体进行CNV形态进行观察和测量。结果:光凝后3 d可见新生血管及走向清晰可见

光凝后7 d CNV达到高峰。结论:用抗体标记Bruch膜。FITC-dextran荧光素灌注小鼠心脏

激光共聚焦显微镜对RPE-脉络膜-巩膜复合体进行氪红激光诱导C57BL/6小鼠的CNV模型进行评估

直观

可靠。

Abstract

Objective: To establish a new assessment method for animal model of choroidal neovascularization(CNV).Methods: In this model

CNV was induced by laser photocoagulation in C57BL/6 mice with the krypton red laser.Animals from each group were sacrificed and were perfused through the heart with fluorescein-labeled dextran(FITC-Dextran) on day 1

and 7 post laser.Retinal pigment epithelium-choroid-scleral tissue

after using a mAb specific for elastin staining

was examined to determine the incidence and size of CNV complex by confocal microscopy.Results: Confocal micrograph of the neovascular complex in C57BL/6 mice showed the new vessels(green)

and damaged Bruch’s membrane(BM) and RPE were stained red for elastin.CNV appeared on day 3 post laser

reaching the peak on day 7 post laser.Conclusion: The new assessment method of animal model of choroidal neovasculation(CNV) is feasible

reliable and visualized.

关键词

脉络膜新生血管动物模型氪红激光共聚焦显微镜评估

Keywords

choroidal neovascularizationanimal modelkrypton red laserconfocal microscopyassessment

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