Objective:To observe the feasibility of constructing the composite skin in vivo whose structure is similar to the skin.Methods:A medical silicone expander was implanted subcutaneously in the back

on both sides symmetrically in each of 10 rabbits.1 w after surgery

fibrous cyst formed around the expander and cultured isogeneic rabbit keratinocytes suspension was injected subcutaneously by the pipeline into the room between fibrous cyst and expander.A control group was set up and the expander was removed after 2 w to see whether the epithelialization could be found on the surface of fibrous cyst by macroscopic and microscopic observation.Results:Transparency of cyst in the experimental group was slightly lower than that of the control group by macroscopic observation

and no other significant difference was found.There was no epithelialization on the surface of fibrous cyst in the control group by microscopic observation whereas structure similar to the epithelializatified on the surface of fibrous cyst in the experiment group could be observed.Conclusion:The composite skin in vivo whose structure is similar to the skin can be constructed with the above

epithelium with the fibrous cyst is feasible for wound coverage.