结核分枝杆菌培养滤液蛋白对鼻息肉患者IL-4、IFN-γ、GM-CSF的影响

邹伟; 朱丽敏; 钱迪; 柯霞; 洪苏玲

doi:10.13406/j.cnki.cyxb.2010.09.044

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结核分枝杆菌培养滤液蛋白对鼻息肉患者IL-4、IFN-γ、GM-CSF的影响

基础研究 | 更新时间：2024-01-17

- 结核分枝杆菌培养滤液蛋白对鼻息肉患者IL-4、IFN-γ、GM-CSF的影响
- Effects of culture filtrate proteins of Mycobacterium tuberculosis on IL-4,IFN-γ,GM-CSF with nasal polyp
- 重庆医科大学学报 2010年35卷第9期页码：1375-1377
- 作者机构：
  
  1. 重庆邮电大学医院耳鼻咽喉科
  2. 重庆医科大学附属第一医院耳鼻喉科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.09.044
  中图分类号： R765.21
- 纸质出版日期：2010，
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[1]邹伟,朱丽敏,钱迪,柯霞,洪苏玲.结核分枝杆菌培养滤液蛋白对鼻息肉患者IL-4、IFN-γ、GM-CSF的影响[J].重庆医科大学学报,2010,35(09):1375-1377.

ZOU Wei, et al. Effects of culture filtrate proteins of Mycobacterium tuberculosis on IL-4,IFN-γ,GM-CSF with nasal polyp[J]. Journal of Chongqing Medical University, 2010,35(9):1375-1377.
[1]邹伟,朱丽敏,钱迪,柯霞,洪苏玲.结核分枝杆菌培养滤液蛋白对鼻息肉患者IL-4、IFN-γ、GM-CSF的影响[J].重庆医科大学学报,2010,35(09):1375-1377. DOI： 10.13406/j.cnki.cyxb.2010.09.044.

ZOU Wei, et al. Effects of culture filtrate proteins of Mycobacterium tuberculosis on IL-4,IFN-γ,GM-CSF with nasal polyp[J]. Journal of Chongqing Medical University, 2010,35(9):1375-1377. DOI： 10.13406/j.cnki.cyxb.2010.09.044.

摘要

目的:观察结核分枝杆菌培养滤液蛋白（Culture filtrate protein

CFP）对鼻息肉（Nasal polyp）患者外周血γ-干扰素（γ-interferon

IFN-γ）、白细胞介素-4（Interleukin-4

IL-4）、粒细胞-巨噬细胞集落刺激因子（Granulocyte-macrophage colony stimulating factor

GM-CSF）3种细胞因子的影响。方法:抽取30例鼻息肉患者外周静脉血

分离单个核细胞（Peripheral blood mononuclear cells

PBMCs）

采用MTT法测定刺激淋巴细胞增殖的最佳CFP浓度

采用酶联免疫吸附试验（Enzyme linked immune sorbent assay

ELISA）检测CFP干预前后PBMCs培养上清液中的IL-4、IFN-γ、GM-CSF水平。结果:鼻息肉患者组PBMCs上清液中IL-4、GM-CSF含量较对照组显著增高

而IFN-γ含量显著减少。CFP干预后鼻息肉患者组上清液IL-4、GM-CSF水平明显降低

而IFN-γ含量显著升高

差异有显著性。结论:CFP能纠正鼻息肉患者体内Th1和Th2细胞因子的失平衡状态

可能成为鼻息肉的一种辅助治疗手段。

Abstract

Objective:To investigate the effect of culture filtrate proteins(culture filtrate proteins

CFP)of Mycobacterium tuberculosis in vitro culture on IL-4

IFN-γ

GM-CSF in patients with nasal polyp.Methods:5 ml peripheral venous bloods was obtained from the enrolled thirty patients with nasal polyp

and peripheral blood mononuclear cells(PBMCs)were isolated.The optimal CFP concentration that stimulated lympholeukoeyte proliferation was measured by MTT.method.IL-4

IFN-γ

GM-CSF levels in culture supematant of the PBMCswere determined by ELISA before and after CFP treatment.Results:Supematant IL-4

GM-CSF levels were all significantly increased

while IFN-γlevel was significantly decreased in these nasal polyp as compared with these normal of contro1.Aftert treatment of CFP

supematant IL-4

GM-CSF levels were significantly reduced

while IFN-γlevel was significantly increased.Conclusion:CFP may inhibit Th2 cell activation and modulate the imbalance state of the Thl and Th2 cytokines.It proves that CFP could be useful in the treatment of nasal polyp.

关键词

鼻息肉结核分支杆菌IL-4IFN-γGM-CSF

Keywords

nasal polypMycohacterium tuberculosisIL-4IFN-γGM-CSF

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