间充质干细胞移植后在溃疡性结肠炎大鼠体内的迁移

段征; 徐艳华; 陈小云; 汪维伟

doi:10.13406/j.cnki.cyxb.2010.08.012

您当前的位置：

首页 >

文章列表页 >

间充质干细胞移植后在溃疡性结肠炎大鼠体内的迁移

基础研究 | 更新时间：2024-01-17

- 间充质干细胞移植后在溃疡性结肠炎大鼠体内的迁移
- Endosomatic transference of transplanted bone marrow mesenchymal stem cells in rat of ulcerative colitis
- 重庆医科大学学报 2010年35卷第8期页码：1152-1155
- 作者机构：
  
  重庆医科大学基础医学院组织胚胎学教研室、干细胞与组织工程研究室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.08.012
  中图分类号： R574.62
- 纸质出版日期：2010，
扫描看全文
[1]段征,徐艳华,陈小云,汪维伟.间充质干细胞移植后在溃疡性结肠炎大鼠体内的迁移[J].重庆医科大学学报,2010,35(08):1152-1155.

DUAN Zheng, et al. Endosomatic transference of transplanted bone marrow mesenchymal stem cells in rat of ulcerative colitis[J]. Journal of Chongqing Medical University, 2010,35(8):1152-1155.
[1]段征,徐艳华,陈小云,汪维伟.间充质干细胞移植后在溃疡性结肠炎大鼠体内的迁移[J].重庆医科大学学报,2010,35(08):1152-1155. DOI： 10.13406/j.cnki.cyxb.2010.08.012.

DUAN Zheng, et al. Endosomatic transference of transplanted bone marrow mesenchymal stem cells in rat of ulcerative colitis[J]. Journal of Chongqing Medical University, 2010,35(8):1152-1155. DOI： 10.13406/j.cnki.cyxb.2010.08.012.

摘要

目的:观察大鼠骨髓间充质干细胞（Bone marrow mesenc hymal stem cells

BMSCs）静脉移植后在体内的迁移。方法:SD大鼠分为A组[免疫结合TNBS/ethanol诱导溃疡性结肠炎（Ulcerative colitis

UC）模型]和B组（正常大鼠）

均尾静脉注射1ml荧光标记的BMSCs。移植后第3、7、14d各处死5只

取材做恒冷切片

荧光显微镜下观察BMSCs在骨髓、肺、肝、脾以及消化道内分布

测定荧光的积分光密度（Integral optical density

IOD）值。结果:BMSCs移植后多分布于血管丰富器官

7d时肺内荧光IOD值最高

14d时骨髓内IOD值最高

但无统计学意义。14d时

A组结肠的IOD值高于同组其余消化道各段以及B组结肠（P<0.05）。结论:静脉移植的BMSCs早期主要分布在血供丰富的器官中

迁移至受损结肠的BMSCs高于正常结肠。

Abstract

Objective:To investigate the transference of transplanted bone marrow mesenchymal stem cells (BMSCs)in rat of ulcerative colitis. Methods:SD rats were randomly distributed into group A colitis induced by immune-combined TNBS/ethanol and group B as control. All rats received caudal vein injection with 1 ml of fluorescence marked BMSCs

then 5 rats were sacrificed at day 3

7 and 14 respectively in each group. Cryostat sections of bone marrow

lung

liver

spleen and gut were made to observe the distribution of BMSCs in these organs

and the fluorimetric integral optical density(IOD)were analyzed. Results:BMSCs were mainly distributed in organs which richs vessels. The highest IOD level of BMSCs in lung was on the 7th day

and was on the 14th day in bone marrow. On the 14th day

the IOD level of BMSCs in colon of group A were higher than those of other segments of digestive tube and also higher than those in group B (P<0.05). Conclusion:Transplanted BMSCs in earlierperiod were mainly distributed in the organs with rich blood supply

and BMSCs migrated to the injured colon are higherthan those of normal colon.

关键词

骨髓间充质干细胞移植溃疡性结肠炎大鼠

Keywords

bone marrow mesenchymal stem cellstransplantationulcerative colitisrat

references

浏览量

217

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

移植骨髓间充质干细胞在溃疡性结肠炎大鼠结肠的分布

硝普钠促进静脉移植BMSCs向溃疡性结肠炎大鼠结肠的迁移

两种溃疡性结肠炎大鼠模型的比较

兔自体骨髓间充质干细胞移植对冷缺血再灌注损伤肾脏细胞增殖与凋亡和肾功能的影响

基于NLRP3/Caspase-1通路探讨薏苡仁多糖对溃疡性结肠炎体外炎症模型的干预作用及机制