FENG Zheng-ping, et al. Influence of different glucose concentrations on the apoptosis of MC3T3-E1 osteoblast[J]. Journal of Chongqing Medical University, 2010,35(6):840-842.
FENG Zheng-ping, et al. Influence of different glucose concentrations on the apoptosis of MC3T3-E1 osteoblast[J]. Journal of Chongqing Medical University, 2010,35(6):840-842. DOI: 10.13406/j.cnki.cyxb.2010.06.021.
Objective:To study the influence of different glucose concentrations on the apoptosis of MC3T3-E1 osteoblast. Methods: Seven culture media of different glucose and mannitol concontrations
5.5
11.1
22.2
33.3 mmol/L were set up. MC3T3-E1 osteolblast was cultured in these media successively for 1
3
7
12 d
and then the percentages of apoptotic cells were measured by flow cytometry. Results:High mannitol can slightly increase the apoptosis of MC3T3-E1 osteoblast
but there was no statistical significance(P>0.05). The inducing action of glucose to the apoptsis of MC3T3-E1 osteoblast was in concentration and time dependent manner(P<0.05
P<0.01). Conclusion:Glucose of elevated concentration significantly promotes the apoptosis of MC3T3-E1 osteoblast.