移植骨髓间充质干细胞在溃疡性结肠炎大鼠结肠的分布

陈晓云; 徐艳华; 姜蓉; 汪维伟

doi:10.13406/j.cnki.cyxb.2010.06.020

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移植骨髓间充质干细胞在溃疡性结肠炎大鼠结肠的分布

基础研究 | 更新时间：2024-01-17

- 移植骨髓间充质干细胞在溃疡性结肠炎大鼠结肠的分布
- Distribution of transplanted bone mesenchymal stem cells in colon of ulcerative colitis rat
- 重庆医科大学学报 2010年35卷第6期页码：845-848
- 作者机构：
  
  重庆医科大学基础医学院组织学与胚胎学教研室重庆市干细胞与组织工程研究室
- 作者简介：
- 基金信息：
  
  重庆医科大学自科预研课题（编号:NSFYY200705）
- DOI：10.13406/j.cnki.cyxb.2010.06.020
  中图分类号： R574.62
- 纸质出版日期：2010，
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[1]陈晓云,徐艳华,姜蓉,汪维伟.移植骨髓间充质干细胞在溃疡性结肠炎大鼠结肠的分布[J].重庆医科大学学报,2010,35(06):845-848.

CHEN Xiao-Yun, et al. Distribution of transplanted bone mesenchymal stem cells in colon of ulcerative colitis rat[J]. Journal of Chongqing Medical University, 2010,35(6):845-848.
[1]陈晓云,徐艳华,姜蓉,汪维伟.移植骨髓间充质干细胞在溃疡性结肠炎大鼠结肠的分布[J].重庆医科大学学报,2010,35(06):845-848. DOI： 10.13406/j.cnki.cyxb.2010.06.020.

CHEN Xiao-Yun, et al. Distribution of transplanted bone mesenchymal stem cells in colon of ulcerative colitis rat[J]. Journal of Chongqing Medical University, 2010,35(6):845-848. DOI： 10.13406/j.cnki.cyxb.2010.06.020.

摘要

目的:观察不同数量大鼠骨髓间充质干细胞（Bone marrow mesenchymal stem cells

BMSC）移植于溃疡性结肠炎（Ulcerative colitis

UC）大鼠模型

探讨BMSC移植的最适数量。方法:分离培养、DAPI荧光标记SD大鼠BMSCs。免疫复合法建立大鼠UC模型

分A、B、C组分别经尾静脉注射1×106、5×106、1×107个DAPI标记的BMSCs。移植后第7、14d各处死5只大鼠

取结肠做恒冷切片

荧光显微镜下记数各组DAPI标记BMSCs在病灶部位和周围正常部位的细胞数。结果:移植7、14d

各组均见BMSCs分布于结肠黏膜

溃疡部位阳性细胞数明显高于非溃疡部位阳性细胞数（P<0.05）。移植7、14d

B组损伤部位的荧光标记细胞多于A组（P<0.05）

但B组与C组间差异不明显。结论:经静脉给UC大鼠移植BMSCs的最适数量为5×106个。

Abstract

Objective :To investigate the optimal dose of transplanted bone marrow mesenchymal stem cells (BMSCs) for the ulcerative colitis rat. Methods:The BMSCs of SD rat were isolated

cultured and labelled with DAPI.Colitis of rat was induced with immunecombined TNBS/ethanol in group A

B.and C. Each rat in 3 groups received caudal vein injection of fluids containing 1×106

5×106

and 1×107 BMSCs respectively. 5 rats in each group were sacrificed at day 7 and 14 after injection.Cryostat sections of colon were prepareted.The numbers of BMSCs in focal area of colitis and surrounding normal tissue were counted under fluorescent microscope. Results:The DAPI marked BMSCs could been seen in the colic mucosa in each group on day 7 and 14. The number of BMSCs in the ulcerated area was bigger than that in the surrounding normal tissue (P<0.05).At day 7 after transplantation

there were more DAPI marked BMSCs in the infected area in group B than in group A(P< 0.05)

but the number did not have significant difference between group B and C.Conclusion :The dose of 5×106 was optimal density of transplanted BMSCs through caudal vein injection in ulcerative colitis rat.

关键词

骨髓间充质干细胞移植溃疡性结肠炎

Keywords

bone marrow mesenchymal stem cellstransplantationulcerative colitis

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