Objective:To establish high efficient transfection and expression system in vitro for phPPARγ1-IRES2-EGFP re- combinant plasmid carrying human peroxisome proliferator-activated receptorγ1(hPPARγ1)gene.Methods:Cationic lipid trans- fection reagent lipofectamine 2000 was used to transfect phPPARγ1-IRES2-EGFP recombinant plasmid and control pIRES2- EGFP plasmid to 293 cells.The profiles of GFP expression and transfection efficiency were measured by fluorescence mi- croscopy.Expression of PPARγ1 in transfected 293 cells were determined by werstern blot and real time quantitative poly- merase chain reaction.Results:GFP expression level was high in transfeced 293 cells

the transfection efficiency of phP- PARγ1-IRES2-EGFP was(83±11)%.In transfected 293 cells

high efficient expression of hPPARγ1 gene were detected both at mRNA and protein levels by real-time PCR and western blot analyses.Conclusion:High efficient transfection and expres- sion system for phPPARγ1-IRES2-EGFP recombinant plasmid in vitro is successfully established

which provide a useful tool in investigating hPPARγgene function as well as establishing molecular platform by which the candidates of unknown hP- PARγligands or activators can be found.