FANG Liaoqiong, et al. Influence on humoral immunity in mice by goat placenta immunoregulatory factor[J]. Journal of Chongqing Medical University, 2005,(3):356-358.
FANG Liaoqiong, et al. Influence on humoral immunity in mice by goat placenta immunoregulatory factor[J]. Journal of Chongqing Medical University, 2005,(3):356-358. DOI: 10.13406/j.cnki.cyxb.2005.03.013.
Objective:To study the humoral immunity effect of goat placenta immunoregulatory factor(GPIF).Methods:Basing on BALB/c mice
the study was carried out by determining the proliferation of B lymphocyte in mice spleen with MTT method
the level of IgM with PFC test
and the level of IgG secreted by mice B cell in vivo and in vitro with ELISA test.Results:GPIF could effeciently increase the level of antibody in vivo.Direct experiment on splenic B lymphocyte
GPIF could also improve the level of antibody in vitro and enable mice lymphocyte induced by LPS to proliferate efficiently.Conclusion:GPIF conld enhance humoral immunity function and recover the ability of humoral immunity in mice with immunodeficiency.