气相色谱法测定脑脊液中乳酸浓度

李崇智; 王其南; 周向阳; 杨健

doi:10.13406/j.cnki.cyxb.1992.03.003

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气相色谱法测定脑脊液中乳酸浓度

更新时间：2024-01-17

- 气相色谱法测定脑脊液中乳酸浓度
- GAS CHROMATOGRAPHIC DETERMINATION OF LACTATE CONCENTRATION IN CEREBROSPINAL FLUID
- 重庆医科大学学报 1992年第3期页码：176-178
- 作者机构：
  
  重庆医科大学第一医院传染科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1992.03.003
  中图分类号：
- 纸质出版日期：1992，
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[1]李崇智,王其南,周向阳,杨健.气相色谱法测定脑脊液中乳酸浓度[J].重庆医科大学学报,1992(03):176-178.

GAS CHROMATOGRAPHIC DETERMINATION OF LACTATE CONCENTRATION IN CEREBROSPINAL FLUID[J]. Journal of Chongqing Medical University, 1992,(3):176-178.
[1]李崇智,王其南,周向阳,杨健.气相色谱法测定脑脊液中乳酸浓度[J].重庆医科大学学报,1992(03):176-178. DOI： 10.13406/j.cnki.cyxb.1992.03.003.

GAS CHROMATOGRAPHIC DETERMINATION OF LACTATE CONCENTRATION IN CEREBROSPINAL FLUID[J]. Journal of Chongqing Medical University, 1992,(3):176-178. DOI： 10.13406/j.cnki.cyxb.1992.03.003.

摘要

本文采用气相色谱法测定脑脊液中的乳酸浓度

脑脊液经酯化处理

再用氯仿提取后

取样5μl上机分析.色谱柱内填料为涂有15%聚二乙二醇丁二酸醋的硅藻土（60—80目）.检测器为FID.恒温分析.10次重复测定批内变异系数为11.33%.平均回收率98.96%

Abstract

This paper reported the determination of lactate concentration in cerebrospinal fluid (CSF) by gas chromatography. CSF was usterified by methyl alcohol

extracted by chloroform

and then a specimen of 5 ul was analyzed by chromatography. Glass column packed with 15% diethylene glycol suecinate on chemalite(60/80mesh) was used. The detector was flame ionization detector. Variance within groups was 11.33% after 10 times of repeated determinations. The recovery rate was 98.96%

关键词

气相色谱法乳酸

Keywords

Gas chromatographyLactate

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