Objective:To observe the influence of tripterygium wilfordii polyglycoside(TWP)on the coagulation function of rats with ulcerative colitis(UC). Methods:Sixty-four female clean grade Sprague-Dawley(SD)rats were randomly divided into 52 model rats and 12 normal control rats. Rats in normal control group were left intact. Rats in model group were used to establish UC rat model by a compound method of 2

4-dinitrochlorobenzene(DNCB) and acetic acid. On the 19 th day

two rats were killed in normal control group and model group

respectively. After the UC rat model being established successfully

UC model rats were randomly divided into5 groups with each group of 10 rats:model control group

heparin treatment control group

TWP low dose group

TWP moderate dose group

TWP high dose group. Rats in normal control group and model control group were not given any drugs. Heparin treatment control group was injected with heparin sodium through caudal vein. TWP low dose group

moderate dose group and high dose group were administrated with TWP 37.5 mg/kg

75 mg/kg and 150 mg/kg

respectively. Rats were given drugs for 15 days. The blood was collected from abdominal aorta;one part was anticoagulated by sodium citrate to detect activated partial thromboplastin time(APTT)

prothrombin time(PT)

fibrinogen(FIB)using automatic blood coagulation analyzer;another part was anticoagulated by ethylene diamine tetraacetic acid(EDTA)to detect platelet(PLT)using automatic blood cell analyzer. Moreover

all rats were killed to collect colon tissue. The morphological and pathological changesof colon tissue were observed. Results:For PT and APTT

TWP low dose group[(7.18±0.22)s

(17.89±0.26)s]

moderate dose group[(7.44 ±0.16) s

(19.87 ±0.46) s] and high dose group [(7.72 ±0.13) s

(22.87 ±1.19) s] were lower than normal control group[(8.75±0.16)s

(24.79±0.77)s](P<0.05)

and higher than model control group[(6.51 ± 0.27)s

(16.82±0.42)s](P<0.05). For FIB and PLT

TWP low dose group[(2.39±0.12)g/L

(1 050.20±28.42)×109/L]

moderate dose group[(2.33±0.12)g/L

(969.60±15.36)×109/L] and high dose group[(1.91±0.06)g/L

(907.60±14.59)×109/L] were higher than normal control group[(1.69±0.12)g/L

(840.80±26.67)×109/L](P<0.05)and lower than model control group[(3.12 ± 0.44)g/L

(1 224.80±75.06)×109/L](P<0.05).Compared with those of model control group

there were less inflammation and lower morphology scoring and ulcer formation grading of colon tissue in rats of drug dose groups. Conclusion:TWP can prolong PT and APTT

and decrease FIB and PLT

thus alleviate inflammation in colon tissues of rats infected with ulcerative colitis.