Objective:To establish a method for the detection of the loop mediated isothermal amplification(LAMP)of mice Sendai virus(Se V). Methods:Using LAMP technology

six specific primers for LAMP were designed with V4 Primer Exploer software

according to the target gene sequence of mice of Se V(gi:9627219)F protein. Se V RNA was extracted with virus RNA kit. Se V specific LAMP(Se V/LAMP)method was established through optimizing the LAMP reaction system. At the same time

validation of specificity

stability

repeatability and sensitivity of the method

and initially clinical testing were performed. Results:The established method of Se V/LAMP can detect RNA Se V specifically

having no reaction with other common pathogens. Se V/LAMP can detect different batches at the same time

or the same batch at different times of RNA Se V. The lower limit of Se V/LAMP detection of Se V RNA was 0.33 pg;the sensitivity of Se V/LAMP detection of Se V RNA was high(respectively 5/30

2/30)compared with that of ELISA detection method. For clinical detection

the positive rate of Se V was 27.5%(11/40). All reactions can be completed within 1 h. Visual experimental results can be achieved by adding calcein intuitive. Conclusion :LAMP method has the advantages of specificity

accuracy

sensitivity

rapid and simple

and therefore can be used for the rapid detection of Se V in mice.