Objective

2

To investigate the feasibility of applying jugular vein blood sampling technique in pharmacokinetic double cycle crossover experiment in conscious rats.

Methods

2

Twenty-four SD rats

half male and half female

were divided into the experimental group and the control group by simplified random method

with 12 rats of half male and half female in each group.Rats in the experimental group were simulated the pharmacokinetic double cycle crossover experiment

with oral administration or tail vein injection of normal saline

0.1 mL of blood was collected by the jugular vein blood sampling technique in conscious rats at different time points before and after administration

and the body weight of the rats was recorded every day.Rats in the control group were normally fed without blood sampling

and their body weights were recorded every day.

Results

2

In the experimental group

blood was collected 11 times per cycle from the jugular vein in every conscious rat

and the total amount of blood collected within 24 hours was 1.1 mL

with the success rate of one-time jugular vein blood collection of 84.1%

and the survival rate in rats of 100%.There was no statistically significant difference in body weight between rats in the experimental group and the control group （

P

>

0.05）.

Conclusion

2

The jugular vein blood sampling technique of conscious rats does not affect the normal growth of rats and can be applied to the pharmacokinetic double cycle crossover experiment

which can meet the requirements of blood collection times and volume for experimental research

and conforms to the "3R" principle of animal welfare.