Objective: To make a convenient and practical in vitro ischemia model by oxygen and glucose deprivation(OGD) in cultured neuronal cells. Methods: The cultured neurons dissociated from the cortices and striata of the neonatal rats were observed on the 14th day after culture. The culture media were exchanged by Earle’s solution with glucose or without glucose

respectively. The group without glucose was placed in a modified chamber(volume

2000ml) and mixture gas (95% N 2

5% CO 2) was put inside. The neuronal injury was detected by the lactate dehydrogenase (LDH) release and the tetrazolium salt 3-(4

5-dimethyl-2-thiazolyl)-2

5 diphenyl-2H tetrazolium bromide (MTT) assay after OGD. Results: The activity of neurons (MTT assay) was decreased and the amount of LDH release from damaged neurons was increased significantly in OGD group than in control (Con) group after reoxygen. Conclusion: Our results suggested that in vitro ischemia model may be induced easily by OGD in cultured neurons

and may be used to further study the mechanism of ischemic neurounal cell injury and its protection.