Objective: In order to pave a way to cure human tumors

we plan to construct expression vector of human endostatin and produce recombinant endostatin from E.coli. Methods: According to the sequence of XVIII collagen gene

cDNA of human endostatin was obtained from the normal liver tissue of a Chinese Han man by RT-PCR . Endostatin cDNA was cloned into PUCm-T vector by T/A cloning technique and sequenced . Then endostatin cDNA was inserted into the PKPL-3a expression vector and the recombined plasmid was transformed into the host strain E.coli POP2136. The endostatin expression was induced by raising the temperature to 42 ℃ and analyzed by SDS-PAGE. Results: Endostatin cDNA sequence encoded 184 amino acid residues. The variation in bases encoding Glu85 and Asp98 was discovered in Chinese. Recombinant human endostatin was expressed as inclusion body and the host expressed a 20kD protein which was confirmed with SDS-PAGE analysis. Conclusion: The cloning and expression vectors of human endostatin were successfully constructed in our laboratory. Expression vector expressed a 20 kD recombined human endostatin at 42℃.