二氧化碳气腹对人卵巢癌细胞系SKOV<sub>3</sub>裸鼠腹腔转移瘤生长的影响及机制探讨

唐均英; 黄金; 姚珍薇

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二氧化碳气腹对人卵巢癌细胞系SKOV₃裸鼠腹腔转移瘤生长的影响及机制探讨

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- 二氧化碳气腹对人卵巢癌细胞系SKOV₃裸鼠腹腔转移瘤生长的影响及机制探讨
- Effects of carbon dioxide pneumoperitoneum on the abdomino-metastatic tumor growth of human ovarian carcinoma cell line（SKOV₃） in nude mice and analysis of its mechanism
- 重庆医科大学学报 2008年第4期页码：441-445
- 作者机构：
  
  1. 重庆医科大学附属第一医院妇产科
  2. 重庆医科大学附属第一医院妇产科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R737.31
- 纸质出版日期：2008，
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[1]唐均英,黄金,姚珍薇.二氧化碳气腹对人卵巢癌细胞系SKOV_3裸鼠腹腔转移瘤生长的影响及机制探讨[J].重庆医科大学学报,2008(04):441-445.

TANG Jun-ying, et al. Effects of carbon dioxide pneumoperitoneum on the abdomino-metastatic tumor growth of human ovarian carcinoma cell line（SKOV₃） in nude mice and analysis of its mechanism[J]. Journal of Chongqing Medical University, 2008,(4):441-445.
[1]唐均英,黄金,姚珍薇.二氧化碳气腹对人卵巢癌细胞系SKOV_3裸鼠腹腔转移瘤生长的影响及机制探讨[J].重庆医科大学学报,2008(04):441-445. DOI：

TANG Jun-ying, et al. Effects of carbon dioxide pneumoperitoneum on the abdomino-metastatic tumor growth of human ovarian carcinoma cell line（SKOV₃） in nude mice and analysis of its mechanism[J]. Journal of Chongqing Medical University, 2008,(4):441-445. DOI：

摘要

目的:研究二氧化碳气腹对卵巢癌细胞裸鼠腹腔转移瘤生长的影响

并初步探讨其机制。方法:构建裸鼠腹腔转移瘤的动物模型

模拟腹腔镜CO2气腹环境

通过观察肿瘤种植生长的情况

及TUNNEL法测定肿瘤组织凋亡情况

以探讨二氧化碳气腹对卵巢癌细胞裸鼠腹腔转移瘤生长的影响;通过免疫组织化学法和Western-blot测定肿瘤组织中VEGF及MMP-9表达

以探讨其可能存在的机制。结果:经CO2气腹处理后

腹腔内肿瘤质量大于对照组

肿瘤组织中VEGF和MMP-9蛋白的表达差异无显著性

各组间肿瘤细胞的凋亡率差异无显著性。结论:CO2气腹有促进卵巢肿瘤细胞SKOV3生长的作用。

Abstract

Objective:To investigate the effects of carbon dioxide(CO2) pneumoperitoneum on the abdomino-metastatic tumor growth of ovarian carcinoma cell in nude mice and to primarily analyze its mechanism.Methods:The nude mice model of abdomino-metastatic tumor of ovarian carcinoma cell was established

and laparoscopic CO2 pneumoperitoneum environment was simulated.Tumor implantation and growth were observed and tumor tissue apoptosis was detected by TUNNEL method

to explore the effect of CO2 pneumoperitoneum on the abdomino-metastatic tumor growth of ovarian carcinoma cell in nude mice.The expressions of VEGF and MMP9 in cancer tissue were determined by immunohistochemistry and Western Blot to analyze the possible mechanism.Results:After the treatment of CO2 pneumoperitoneum

the tumor quality in abdominal cavity was higher than that of the control group

the expression difference of VEGF and MMP9 in tumor tissues was not significant and tumor cell apoptosis rates of each group do not differ significantly.Conclusions:Carbon dioxide pneumoperitoneum can promote the growth of ovarian carcinoma cell SKOV3.

关键词

CO2气腹裸鼠凋亡VEGFMMP-9

Keywords

Carbon dioxide pneumoperitoneumnude miceapoptosisVEGFMMP-9

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二氧化碳气腹对人卵巢癌细胞系SKOV3裸鼠腹腔转移瘤生长的影响及机制探讨

Effects of carbon dioxide pneumoperitoneum on the abdomino-metastatic tumor growth of human ovarian carcinoma cell line（SKOV3） in nude mice and analysis of its mechanism

DOI：

摘要

Abstract

关键词

Keywords

references

二氧化碳气腹对人卵巢癌细胞系SKOV₃裸鼠腹腔转移瘤生长的影响及机制探讨

Effects of carbon dioxide pneumoperitoneum on the abdomino-metastatic tumor growth of human ovarian carcinoma cell line（SKOV₃） in nude mice and analysis of its mechanism