Objective:To study the effects of CO

2

pneumoperitoneum on the signal transduction pathways of human cervical carcinoma cell line CasKi. Methods:The imitated CO

2

pneumoperitoneum model was established in vitro. After CasKi cells were exposed to CO

2

pneumoperitoneum at 12

13 or 14mmHg for 2h

the cell growth and expressions of phosphor-ERK and phosphor-Akt were examined by MTT

immunocytochemistry and Western blotting respectively. The untreated CasKi cells served as control. Results:After treatment of CO

2

pneumoperitoneum

the proliferation of human cervical carcinoma cell line CasKi significantly increased

and the expressions of phosphor-ERK and phosphor-Akt also significantly increased（P

<

0.01）. Among the CO

2

treated groups

the proliferation of human cervical carcinoma cell line CasKi and the expressions of phosphor-ERK and phosphor-Akt were not observed significant difference（P

>

0.05）. Conclusion:Under the pressure and during the exposure time which were often used in operational laparoscopy

CO

2

pneumoperitoneum could activate correlated signal transduction pathways

which may be one reason for the promoting effect of CO

2

pneumoperitoneum on malignant cells growth.