Objective:To clone human APOEε3 gene for construction of mammalian expression vector pEGFP-N1-APOEε3. Methods:The cDNA encoding APOEε3 was cloned by the methods RT-PCR from total human embryonic brain RNA. The gene was ligated with PMD19-T vector and then was excised from PMD19-T-APOEε3 plasmid and inserted into pEGFP-N1 to construct eukaryotic expression vector. The expression of APOEε3 gene was identified by western-blot. Results:APOEε3 gene sequence and vector were identified correctly by enzyme digestion and sequence analysis. The APOEε3 gene was transfected into HEK293 cells and expressed successfully. Conclusion:The APOEε3 gene is successfully cloned and its eukaryotic expression vector is obtained

which provides basis for the further experimental researches on APOE functions.