Objective: To observe the influencing factors for efficiency of liposome-mediated transfection into human umbilical vein endothelial cell(HUVEC). Methods: Transferring HUVECs by distinct conditions

such as ratios of liposome and plasmid

densities of per well and different times of incubation

and transfection rates were observed and calculated by fluorescent microscope and flow cytometry. Results:⑴ When density of per well exceeded 2×l04 and times exceeded 4 hours

liposome-mediated transfection efficiency of endothelial cells decreased. ⑵With adding plasmid quality

transfection rates increased;while plasmid quality exceeded 1.0 μg

in condition of 2×l04 per well and liposome volume 2 μl

transfection efficiency reached the peak level. ⑶With adding liposome volume

transfection rates increased;while liposome volume was 8 μl

in condition of 2×104 per well and plasmid quality 1 μg

transfection efficiency was depressed.When ratios of liposome and plasmid were 1:6～1:8

the optimal transfection efficiency was 18.62%. Conclusion: Using optimal transfection parameter

we obtained the optimal transfection efficiency .