细粒棘球绦虫重组pCD-Eg95质粒构建及鉴定

周辉; 李文桂

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细粒棘球绦虫重组pCD-Eg95质粒构建及鉴定

基础研究 | 更新时间：2024-01-17

- 细粒棘球绦虫重组pCD-Eg95质粒构建及鉴定
- Construction and identification of the recombinant pCD-Eg95 plasmid of Echinococcus granulosus
- 重庆医科大学学报 2009年34卷第1期页码：13-15
- 作者机构：
  
  重庆医科大学附属第一医院传染病寄生虫病研究所
- 作者简介：
- 基金信息：
  
  国家自然科学基金（30671835）
- DOI：
  中图分类号： R383.33
- 纸质出版日期：2009，
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[1]周辉,李文桂.细粒棘球绦虫重组pCD-Eg95质粒构建及鉴定[J].重庆医科大学学报,2009,34(01):13-15.

ZHOU Hui, et al. Construction and identification of the recombinant pCD-Eg95 plasmid of Echinococcus granulosus[J]. Journal of Chongqing Medical University, 2009,34(1):13-15.
[1]周辉,李文桂.细粒棘球绦虫重组pCD-Eg95质粒构建及鉴定[J].重庆医科大学学报,2009,34(01):13-15. DOI：

ZHOU Hui, et al. Construction and identification of the recombinant pCD-Eg95 plasmid of Echinococcus granulosus[J]. Journal of Chongqing Medical University, 2009,34(1):13-15. DOI：

摘要

目的:构建并鉴定细粒棘球绦虫（Echinococcus granulosus

Eg）重组pCD-Eg95质粒。方法:从细粒棘球蚴包囊中分离原头节

超声粉碎后抽提总RNA

采用RT-PCR方法扩增Eg95编码基因

将该基因定向克隆到真核表达载体pCDNA3.1中构建pCD-Eg95重组质粒;电穿孔转化大肠杆菌BL21（DE3）株

抽提质粒进行酶切及PCR鉴定。结果:电泳及测序证实471bpEg95基因克隆成功。经酶切及PCR证实重组质粒pCD-Eg95成功转入BL21（DE3）。结论:成功构建了Eg重组pCD-Eg95质粒。

Abstract

Objective: To construct and identify the recombinant pCD-Eg95 plasmid of Echinococcus granulosus. Methods: Total RNA was extracted from hydatid cyst protoscoleces shattered by supersound. The Eg95 coding gene was amplified by RT-PCR

and then was cloned into the eukaryotic expression vector pCDNA3.1 to construct the recombinant pCD-Eg95 plasmid. The recombinant plasmid was electroporated into E. coliBL21(DE3) strain. Results: The 471 bp Eg95 coding gene was successfully amplified

and the recombinant pCD-Eg95 plasmid was successfully constructed

which was confirmed by restriction endonuclease digestion and PCR. Conclusion: The results could provide the basis for the further research of the nucleic acid vaccine of Echinococcus granulosus.

关键词

细粒棘球绦虫重组pCD-Eg95质粒构建鉴定

Keywords

Echinococcus granulosusRecombinant pCD-Eg95 plasmidconstructionidentification

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