Objective: To construct an adenovirus vector encoding mouse PGC1α gene for further study on its function. Methods: Recombinant shuttle plasmid pAdTrack-CMV-PGC1α was linearized by PmeⅠ and transformed into Escherichia coli BJ5183 carrying backbone plasmid pAdeasy-1 to obtain adenovirus plasmid through homologous recombination. The recombinant adenovirus plasmid was digested by PacⅠ and transfected into HEK293 cells to form adenvirus particles. The virus titer was determined by TCID50 method. The expression of PGC1α in 293 cells was detected by Western blot. Results: The packaging cell showed typical CPE

and the virus titer was about 3×1010 TCID50/ml after amplification. The expression of PGC1α mediated by the recombinant adenovirus vector was verified by Western blot. Conclusion: The adenovirus vector encoding mouse PGC1α gene was successfully constructed

and the protein was observed in 293 cell.