Objective:To investigate the effects of Shenfu injection(SFI)on the permeability of blood brain barrier(BBB)and the nervous function of focal cerebral ischemic-reperfusion injured rats

and judge if SFI has protective effects for focal cerebral ischemic-reperfusion injured rats to provide theoretical proof for the clinical application of SFI.Methods:Eighty male SD rats were divided into four groups randomly:group A(the sham operated control group)

group B(the sham operated trial group)

group C(the control group)and group D(the trial group).The rats in group A and group C received i.v.a bolus of normal saline(NS)for 10 mg/kg 5 min after anesthesia

while those in group B and group D received a bolus of SFI for 10 mg/kg instead.The focal cerebral ischemic-reperfusion injury model was established with thread embolism of middle cerebral artery(MCA)according to Zea Longa’s method in group C and group D.The time for postanesthetic revival in each group was recorded.The Longa score in each group was recorded at 2 h of occlusion and 24 h of reperfusion.The injury to the blood-brain barrier was observed by the content of Evan’s blue(EB)in the cerebral tissue

the changes of the structure of BBB were observed with electron microscope at the end of the experiment.Results:The body weight in each group had no statistical difference.The time for revival from anesthesia in group A and group B was shorter than that in group C and group D(P<0.05)

and that in group C was longer than that in group D(P <0.05)but there was no difference between group A and group B.After ischemia for two hours

Longa score in group A and group B was lower than that in group C and group D(P<0.01)

and there was no difference either between group A and group B

group C and group D.After reperfusion for 24 h

Longa score in group A and group B was lower than thatin group C and group D

there was no difference between group A and group B(P<0.01)

and Longa score in group C was higher than that in group D(P<0.01).At the 24th hour of reperfusion

Longa score in group C was higher than that at the 2nd hour of ischemia(P<0.01)

but there was no difference for group D.After ischemia for 2 h and reperfusion for 24 h

the rats in group A and group B had an evidently lower Evan’s blue content in the cerebral tissue than that of the rats in group C and group D

and there was no differe ncebetween group A and group B

while that of the rats in group C was higher than that of the rats in group D.Under the electron microscope

brain edema and microvessel pressed in group C were more severe compared with those in group D.But there was no significant abnormality in group A and group B.Conclusion:Shenfu injection could reduce blood-brain barrier permeability after focal cerebral is-chemic-reperfusion injury and it has significant nervous function protection against ischemic-reperfusion damage.