Objective:to investigate the insulin signal transduction protein in the adipocytes of Type 2 diabetics:Insulin Receptor Substrate-1(IRS-1)tyrosine phosphorylation and Protein Kinase B(PKB/AKT) Ser473 phosphorylation level and expression of microinflammatory cytokine interleukin-6(IL-6).Methods:Usingprimary cell culture

adipocytes from abdominal subcutaneous adipose tissue of Type 2 diabetics(Type 2 diabetes group) and non-diabetics(non-diabetes control group)were cultured.IRS-1 tyrosine phosphorylation and AKT-Ser473 phosphorylation level in the adipocytes of the two groups with insulin stimulation was detected by the Immunoprecipitation and western blot.IL-6 level in adipocytes culture supernatant was evaluated by ELISA.Results:(1) The IRS-1 tyrosine phosphorylation and AKT-Ser473 phosphorylation level in the adipocytes with insulin stimulation of Type 2 diabetes group were significantly lower than that of the non-diabetes control group(P<0.05);(2)The IL-6 protein level in the adipocytes medium of Type 2 diabetes group was significantly higher than that of the non-diabetes control group(P<0.05).Conclusion:The adipocytes of Type 2 diabetics present insulin resistance and microinflammatory states.