Objective To investigate the effects of kaempferol on the expression of α-smooth muscle actin (α-SMA)，interleukin-2(IL-2)，interleukin-6 (IL-6) and interferon-γ (IFN-γ) in skin scar rats. Methods Thirty healthy SD rats were selected and divided into three groups，10 rats in each group，namely blank group，model group and treatment group.Rats in blank group were not treated，while rats in model group and treatment group were modeled.In the treatment group，kaempferol solution was applied around the wound.After 7 days，the area of skin injury and scar in rats were observed.The levels of IL-2，IL-6 and IFN-γ were measured by ELISA. Results After intervention，the thickness of dermis and epidermis in the treatment group was significantly less than that in the model group，and the collagen arrangement was more regular.In the immunohistochemical staining images of skin tissues of rats in the three groups，the positive expression of α-SMA protein in the treatment group was significantly lower than that in the model group (P<0.05).The expression of α-SMA protein in the treatment group was significantly lower than that in the model group (P<0.05).The levels of IL-2 and IFN-γ in the skin tissue of rats in the treatment group were significantly higher than those in the model group，and the level of IL-6 was significantly lower than that in the model group (P<0.05).Conclusion Kaempferol has a certain therapeutic effect on skin scar rats.It can reduce the scar area，decrease the positive expression rate of α-SMA and improve the inflammatory reaction.