Objective To investigate the effect of different concentrations of Nepal Dock Root decoction on the relief of mice with primary liver cancer. Methods Mice were randomly divided into 5 groups:negative control group

positive control group and nepal dock root decoction groups included high dose group（300 mg/kg）

medium dose group（240 mg/kg） and low dose group（200 mg/kg） .The experimental group established a liver cancer H22 tumor model

and used gavage to observe the effect of nepal dock root decoction on tumor mass and survival cycle of H22 tumorbearing mice.The content of TNFα and VEGF in peripheral blood were determined by ELISA.HE staining were taken to observe the morphology of tumor cells.Immunohistochemistry was used to detect tumor cell apoptosis and cell cycle.The spleen index was calculated to observe the immune system. Results Nepal dock root decoction significantly prolonged the survival of H22 tumorbearing mice and inhibited tumor growth (P<0.01)

which promoted cancer cell apoptosis and shortened cancer cell cycle.With the increase of the dosage of rhubarb decoction

the tumor quality gradually decreased and the inhibitory rate gradually increased

the difference between groups was statistically significant（P＜0.05）. Conclusion Different concentrations of nepal dock root decoction have certain inhibitory effects on primary liver cancer

and the intensity is related to concentration.