Objective To investigate the application of diffusion tensor imaging（DTI）on normal aging brain tissue by comparing average diffusion coefficient（ADC）and fraction anisotropy（FA）of the young and middle-elder people via high field magnetic resonance equipment.Methods Sixty healthy volunteers who underwent Magnetic Resonance Imaging（MRI）in brain imaging center of Southwest University from September 2011 to January 2012 were selected as study objects and were equally divided into the young group（20-30 years old）and the middleelder group（40-59 years old）.All study objects experienced DTI and ADC and FA value of different area of interest（ROI）were gained.Results ADC values of study objects with different gender but same ROI had no statistically significant differences（P>0.05）

and FA values of study objects with different gender but same ROI had no statistically significant differences（P>0.05）.Comparison of ADC values in male of different age groups showed that ADC values of other ROI had statistically significant differences（P<0.05）

but except left thalamus

bilateral cerebrospinal fluid and bilateral occipital cortex.Comparison of ADC values in female of different age groups showed that ADC values of other ROI had statistically significant differences（P<0.05）

but except right thalamus

bilateral cerebrospinal fluid and bilateral occipital cortex.Comparison of FA values in male of different age groups showed that FA values of other ROI had statistically significant differences（P<0.05）

but except right thalamus

bilateral cerebrospinal fluid and bilateral occipital cortex.Comparison of FA values in female of different age groups showed that FA values of other ROI had statistically significant differences（P<0.05）

but except bilateral thalamus

bilateral cerebrospinal fluid and bilateral occipital cortex.Conclusion DTI is a sensitive equipment which is noninvasive and can demonstrate the abnormal white matter fiber tracts

reflecting the change of brain minute structure according to changes of FA and ADC value.Therefore

it is beneficial to study the normal pre-aging and has significance to prevent and early diagnose the geratic cerebral diseases caused by normal aging.