Hydrogen peroxide (H2O2) is one of the important reactive oxidative species in cells，which plays a role in cell differentiation，proliferation，immunity，signal transduction and so on. Therefore，accurate analysis and detection of H2O2 is of great significance. Fluorescence detection technology is widely used to monitor H2O2，because of its advantages of simple operation，high biocompatibility，high sensitivity，high selectivity and real-time in-situ detection. Organic small molecule fluorescent probes for H2O2 have been intensively studied，regarding to its good membrane permeability and easy structure tuning. In this paper，some research in this field in the past five years was summarized，based on the response mechanism of small organic molecule fluorescent probes for H2O2. The design，construction and biological application of H2O2 fluorescent probe were also discussed. And the development direction and application prospect of H2O2 fluorescent probe were prospected.