Objective To analyze the relationship between interleukin-22（IL-22）and RAD50 gene polymorphisms and suscep⁃ tibility in children with asthma.Methods 76 children with asthma admitted to our hospital from January 2019 to January 2021 were se⁃ lected as the observation group，and 62 healthy children who underwent physical examination during the same period were were selected as the control group.The background information of the two groups was collected.IL-22（rs17224704，rs2227473）and RAD50 （rs6871536，rs17166050）gene polymorphisms were detected using polymerase chain reaction and direct sequencing.Regression analy⁃ sis was performed to identify factors influencing the occurrence of childhood asthma.Results There were statistically significant differ⁃ ences between the two groups in terms of birth method，antibiotic use frequency before the age of one，pet ownership，family history of al⁃ lergies，family history of asthma，and passive smoking（P < 0.05）.The two groups showed statistically significant differences in the al⁃ lele frequencies of rs17224704 and rs2227473 on IL-22 gene，as well as in the genotype and allele frequencies of rs6871536 and rs17166050 on RAD50 gene（P < 0.05）.There was an interaction effect between rs17224704 and rs2227473（P < 0.05）.Logistic re⁃ gression analysis found that children with a family history of allergies or asthma had an increased risk of asthma（P < 0.05）.Children carrying the A allele at rs17224704 on IL-22 gene had an increased risk of asthma compared to those carrying the T allele（P < 0.05），while children carrying the C allele at rs6871536 on RAD50 gene had an increased risk of asthma compared to those carrying the T al⁃ lele（P < 0.05）.Conclusion The polymorphisms of rs17224704 on IL-22 gene and rs6871536 on RAD50 gene were closely associated with susceptibility to childhood asthma.