Objective To examine the effect of management by objectives combined with management by post access in the improvement of the work capabilities of newly recruited nursing staff in the tripartite cooperative health examination center of large public hospitals. Methods Sixty nurses（26 guidance and testing positions and 34 nursing positions）with over 2 years of clinical nursing experience，who were newly recruited to the tripartite co⁃operative health examination center of large public hospitals，were included in this study. Thirty nurses recruited be⁃fore August 2019 were assigned to the control group for routine management，and 30 nurses recruited after August 2019 were assigned to the study group for compound management by objectives+management by post access+rou⁃tine management. Each group contained 13 guidance and testing positions and 17 nursing positions. The theoretical and practical assessments，excellence rate，and capabilities of the nursing staff were compared between the two groups. Results The study group had significantly better theoretical and practical scores，excellence rate，and ca⁃pabilities（etiquette，communication，service，and health promotion and education）than the control group（all P<0.05）. Conclusion The application of management by objectives combined with management by post access for newly recruited nurses in the tripartite cooperative health examination center is favorable for improving the profes⁃sional skills，excellence rate，capabilities，and service qualities of nurses.