Objective To prepare phase-shifted folate-modified and HCPT-loaded nanodroplets（FA-HCPT-Fe

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-PFP NDs）

and to investigate the properties and multimodality imaging in vitro.Methods were prepared through the rotary evaporation and sonication process.The size and the stability of FA-HCPT-Fe

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-PFP NDs were detected at different time point after preparation.The phase transition characteristic of the nanodroplets was detected.Drug loading was determined with reverse-phase high-performance liquid chromatographic system. Targeting efficiency of FA-HCPTFe

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-PFP NDs was validated with cell experiment.Photoacoustic and magnetic resonance imaging were performed on the gel blocks prepared by different concentrations of nanoparticles. Ultrasonic imaging was performed after irradiation of the nanoparticle emulsion with LIFU.The above experiments were used to verify the ability of nanoparticles to enhance photoacoustic imaging

MRI and ultrasound imaging.Results and encapsulation efficiency was（60.51±2.33）%.Phase-transition was observed of FA-HCPT-Fe

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-PFP NDs with elevated temperature.Targeting experiments in vitro confirmed that the nanoparticles had good targeting ability to SKOV3 tumor cells rich in folate receptors. FA-HCPT-Fe

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-PFP NDs nanoemulsions showed obvious photoacoustic signal

MRI signal and ultrasound signal in vitro.Conclusion molecular probe with great potential.