The rapid development of gene transfection technology has not only revolutionized the research of many basic problems in biology and medicine

but also promoted the progress of diagnosis and treatment.As a biologically active macromolecule of nucleic acids

the gene is easily degraded in the body

so the carrier for gene delivery is particularly important.Ultrasound-targeted microbubble destruction technology（UTMD） utilizes the interaction between ultrasound and microbubbles and the biological effects it produces to enable microbubbles to carry genes for gene transfection and can be used in combination with a variety of vectors.The treatment of a variety of diseases provides an effective means.This article reviews the recent advances in gene transfection by ultrasound-targeted microbubble destruction.