Objective To investigate the feasibility of layer-specific myocardial strain based on echocardiography in evaluating subclinical myocardial injury in patients with bicuspid aortic valve.Methods One hundred and four patients with bicuspid aortic valve and normal LVEF were selected

they were divided into normal valve function group with 44 patients（group A） and abnormal valve function group with 60 patients（group B） according to whether valve dysfunction occurred.In addition

50 healthy volunteers were selected as control group.The general clinical data

conventional ultrasound parameters and layer-specific myocardial strain parameters were compared.Twenty patients were randomly selected to analyze inter-and intraobserver coherence.Results The average age in group B was significantly higher than that in group A

and the difference was statistically significant（P<0.05）.The average LVIDd and AAO in group B were significantly higher than those in group A and the control group

and the differences were statistically significant（all P<0.05）.LSavg

LSepi and LSendo in group A were significantly lower than those in the control group

and the differences were statistically significant（all P<0.05）.LSavg

LSepi

LSmid

CSavg

CSepi and CSmid in group B were significantly lower than those in the control group and group A

and the differences were statistically significant（all P<0.05）.LSendo and CSendo in group B were significantly lower than those in the control group

and the differences were statistically significant（all P<0.05）.The intra-and inter-observer LOA of the longitudinal strain peak and circumferential strain peak in all layers of left ventricular myocardium evaluated by layer-specific myocardial strain were-6.0%～5.0%

and the intra-class correlation coefficient were≥0.80.Conclusion Layer-specific myocardial strain based on echocardiography can be used to assess the cardiac cycle longitudinal peak strain of the left ventricular outer and inner layer in patients with bicuspid aortic valve and normal LVEF

which is beneficial to the early detection of subclinical myocardial injury.