lncRNA JPX靶向miR-378参与丹参酮ⅡA对结肠癌SW620细胞增殖、克隆、凋亡的影响

钱峻; 曹宜; 李灵常; 余佳霖; 霍介格

doi:10.16016/j.1000-5404.202011068

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lncRNA JPX靶向miR-378参与丹参酮ⅡA对结肠癌SW620细胞增殖、克隆、凋亡的影响

基础医学 | 更新时间：2021-08-02

- lncRNA JPX靶向miR-378参与丹参酮ⅡA对结肠癌SW620细胞增殖、克隆、凋亡的影响
- Mechanism of lncRNA JPX targeting miR-378 in tanshinone ⅡA affecting proliferation, cloning and apoptosis in colon cancer SW620 cells
- 陆军军医大学学报 2021年43卷第9期页码：837-844
- 作者机构：
  
  1. 南京中医药大学中医学院中西医结合学院中医诊断教研室
  2. 南京中医药大学中医药文献研究所本草文献研究室
  3. 南京中医药大学附属中西医结合医院肿瘤科
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.202011068
  中图分类号： R394.2;R735.35;R285.5
- 纸质出版日期：2021，
  
  网络出版日期：2021-5-15，
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[1]钱峻,曹宜,李灵常,等.lncRNA JPX靶向miR-378参与丹参酮ⅡA对结肠癌SW620细胞增殖、克隆、凋亡的影响 [J].陆军军医大学学报(原第三军医大学学报),2021,43(09):837-844.QIAN Jun,CAO Yi,LI Lingchang,et al.Mechanism of lncRNA JPX targeting miR-378 in tanshinone ⅡA affecting proliferation, cloning and apoptosis in colon cancer SW620 cells[J].J Amry Med Univ (J Third Mil Med Univ),2021,43(09):837-844.

QIAN Jun, CAO Yi, LI Lingchang, et al. Mechanism of lncRNA JPX targeting miR-378 in tanshinone ⅡA affecting proliferation, cloning and apoptosis in colon cancer SW620 cells[J]. Journal of Army Medical University, 2021,43(9):837-844.
[1]钱峻,曹宜,李灵常,等.lncRNA JPX靶向miR-378参与丹参酮ⅡA对结肠癌SW620细胞增殖、克隆、凋亡的影响 [J].陆军军医大学学报(原第三军医大学学报),2021,43(09):837-844.QIAN Jun,CAO Yi,LI Lingchang,et al.Mechanism of lncRNA JPX targeting miR-378 in tanshinone ⅡA affecting proliferation, cloning and apoptosis in colon cancer SW620 cells[J].J Amry Med Univ (J Third Mil Med Univ),2021,43(09):837-844. DOI： 10.16016/j.1000-5404.202011068.

QIAN Jun, CAO Yi, LI Lingchang, et al. Mechanism of lncRNA JPX targeting miR-378 in tanshinone ⅡA affecting proliferation, cloning and apoptosis in colon cancer SW620 cells[J]. Journal of Army Medical University, 2021,43(9):837-844. DOI： 10.16016/j.1000-5404.202011068.

摘要

目的研究lncRNA JPX靶向miR-378参与丹参酮ⅡA对结肠癌SW620细胞增殖、克隆、凋亡的影响及机制。方法结肠癌SW620细胞分成对照组、丹参酮ⅡA组（丹参酮ⅡA处理）、vector组（转染阴性对照载体）、JPX组（转染pcDNA-JPX）、丹参酮ⅡA+vector组(转染阴性对照载体，然后给予丹参酮ⅡA处理）、丹参酮ⅡA+JPX组（转染pcDNA-JPX，然后给予丹参酮ⅡA处理）、丹参酮ⅡA+JPX+miR-NC组（共转染pcDNA-JPX、mimics control，然后给予丹参酮ⅡA处理）、丹参酮ⅡA+JPX+miR-378组（共转染pcDNA-JPX、miR-378 mimics，然后给予丹参酮ⅡA处理）、丹参酮ⅡA+Anti-NC组（转染inhibitor control，然后给予丹参酮ⅡA处理）、丹参酮ⅡA+Anti-miR-378组（转染miR-378 inhibitor，然后给予丹参酮ⅡA处理）。CCK-8检测细胞增殖，平板克隆实验检测细胞克隆形成能力，流式细胞术检测细胞凋亡。荧光素酶报告系统检测定JPX和miR-378的靶向关系。结果与对照组比较，丹参酮ⅡA组结肠癌SW620细胞存活率、克隆形成数目均降低，凋亡率升高（P<0.05）。与vector组比较，JPX组结肠癌SW620细胞存活率、克隆形成数目均升高，凋亡率降低（P<0.05）。与丹参酮ⅡA+vector组比较，丹参酮ⅡA+JPX组结肠癌SW620细胞存活率、克隆形成数目均升高，凋亡率降低（P<0.05）。与丹参酮ⅡA+Anti-NC组比较，丹参酮ⅡA+Anti-miR-378组结肠癌SW620细胞存活率、克隆形成数目均升高，凋亡率降低（P<0.05）。与丹参酮ⅡA+JPX+miR-NC组比较，丹参酮ⅡA+JPX+miR-378组结肠癌SW620细胞存活率、克隆形成数目均降低，细胞凋亡率升高（P<0.05）。JPX靶向促进miR-378表达。结论丹参酮ⅡA通过下调JPX靶向miR-378诱导结肠癌SW620细胞凋亡。

Abstract

ObjectiveTo study the mechanism of lncRNA JPX targeting miR-378 in the proliferation

cloning and apoptosis of colon cancer SW620 cells induced by tanshinone ⅡA. MethodsColon cancer SW620 cells were divided into control group

tanshinone ⅡA treatment group

vector group(transfected with negative control vector)

JPX group(transfected with pcDNA-JPX)

tanshinone ⅡA+vector group(transfected with negative control vector followed by tanshinoneⅡA treatment)

tanshinoneⅡA+JPX group(transfected with pcDNA-JPX followed by tanshinoneⅡA treatment))

tanshinoneⅡA+JPX+miR-NC group(cotransfected with pcDNA-JPX and mimics control

followed by tanshinoneⅡA treatment)

tanshinoneⅡA+JPX+miR-378 group(cotransfected with pcDNA-JPX and miR-378 mimics

then treated with tanshinoneⅡA)

tanshinoneⅡA+anti-NC group(transfected with inhibitor control followed by tanshinoneⅡA treatment)

tanshinoneⅡA+anti-miR-378 group(transfected with miR-378 inhibitor followed by tanshinoneⅡA treatment). CCK-8 assay was used to detect cell proliferation

plate cloning to observe clone formation ability

and flow cytometry to measure cell apoptosis. Luciferase reporter system was adopted to study the targeting relationship between JPX and miR-378. ResultsCompared with the control group

the cell survival rate and number of formed cell clones were reduced

and the apoptotic rate was increased in the SW620 cells from the tanshinoneⅡA group(P<0.05). But the opposite results were seen when the JPX group was compared with the vector group(P<0.05)

so were between the tanshinone ⅡA+JPX group and the tanshinone ⅡA+vector group(P<0.05)

and between the tanshinone ⅡA+Anti-miR-378 group and the tanshinone ⅡA+Anti-NC group(P<0.05). The cell survival rate and number of formed cell clones were reduced

and the apoptotic rate was increased in the tanshinone ⅡA+Anti-miR-378 group than the tanshinone ⅡA+JPX+ miR-NC group(P<0.05). JPX targeted to promote miR-378 expression. ConclusionTanshinone ⅡA induces apoptosis in colon cancer SW620 cells by down-regulating JPX targeting miR-378.

关键词

丹参酮ⅡA结肠癌miR-378JPX凋亡

Keywords

tanshinone ⅡAcolon cancermiR-378JPXapoptosis

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