初生至成年大鼠膀胱ICC细胞增殖变化及其与膀胱兴奋性的关系

郑俊; 付坚; 熊太林; 杨超; 张恒; 吴小军; 沈文浩; 周占松

doi:10.16016/j.1000-5404.201608150

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初生至成年大鼠膀胱ICC细胞增殖变化及其与膀胱兴奋性的关系

基础医学 | 更新时间：2020-08-05

- 初生至成年大鼠膀胱ICC细胞增殖变化及其与膀胱兴奋性的关系
- Proliferation pattern of ICCs in rat bladder from newborns to adults and its relationship with bladder excitability
- 第三军医大学学报 2017年39卷第2期页码：175-178
- 作者机构：
  
  400038 重庆, 第三军医大学西南医院全军泌尿外科中心
- 作者简介：
  
  周占松, 电话: (023)68754186, E-mail: zhouzhansong@sohu.com Zhou Zhansong, Tel: (023)68754186, E-mail: zhouzhansong@sohu.com
- 基金信息：
  
  国家自然科学基金面上项目(81100495)
- DOI：10.16016/j.1000-5404.201608150
  中图分类号： R322.62;R329.28;R334.3
- 纸质出版日期：2017-1-30，
  
  收稿日期：2016-8-1，
  
  录用日期：2016-9-25
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郑俊, 付坚, 熊太林, 等. 初生至成年大鼠膀胱ICC细胞增殖变化及其与膀胱兴奋性的关系[J]. 第三军医大学学报, 2017,39(2):175-178.

Jun Zheng, Jian Fu, Tailing Xiong, et al. Proliferation pattern of ICCs in rat bladder from newborns to adults and its relationship with bladder excitability[J]. J Third Mil Med Univ, 2017,39(2):175-178.
郑俊, 付坚, 熊太林, 等. 初生至成年大鼠膀胱ICC细胞增殖变化及其与膀胱兴奋性的关系[J]. 第三军医大学学报, 2017,39(2):175-178. DOI： 10.16016/j.1000-5404.201608150.

Jun Zheng, Jian Fu, Tailing Xiong, et al. Proliferation pattern of ICCs in rat bladder from newborns to adults and its relationship with bladder excitability[J]. J Third Mil Med Univ, 2017,39(2):175-178. DOI： 10.16016/j.1000-5404.201608150.

摘要

目的

探讨初生至成年时期大鼠膀胱Cajal间质细胞(interstitial cells of Cajal，ICC)增殖变化情况及其与相应时间点膀胱兴奋性的关系。

方法

40只刚出生的大鼠分为2组，每组20只。第1组从出生后第1、15、30、45、60天对其膀胱进行c-kit和Ki67染色，观察ICC细胞的增殖情况；第2组在上述同一时间对其膀胱进行c-kit和SM-actin染色，共聚焦显微镜下观察ICC的分布密度，并测定同期膀胱肌条的兴奋性。

结果

膀胱ICC细胞的增殖速度和分布密度从第1天开始逐步增加，到第30天后增殖速度明显下降，分布密度也随之趋于稳定。大鼠逼尿肌肌条兴奋性从出生后第1天逐渐下降，第 45～60天趋于稳定。

结论

大鼠膀胱ICC细胞在出生后迅速增殖，到第45天分布密度趋于稳定。膀胱兴奋性随着ICC分布密度的增加逐渐降低，并趋于稳定。

Abstract

Objective

To observe the proliferation pattern of interstitial cells of Cajal (ICC) in the bladder of rats from birth to adulthood and explore its relationship with bladder excitability.

Methods

Forty newborn rats were divided into 2 groups

with 20 rats in each group. The bladder from the first group underwent c-kit and Ki67 staining on days 1

45 and 60 after birth to observe cell proliferation. The bladder from the second group underwent c-kit and SM-actin staining to observe distribution density of ICCs under a confocal microscope

and the excitability of bladder muscle strips was measured at the same time points.

Results

The proliferation and distribution density of ICCs in the bladder started to increase since day 1 and culminated on day 30. Then the proliferation significantly slowed down

and the distribution density tended to be stable. The excitability of bladder muscle stripes was in a decrease trend from day 1

and then turned to stable from day 45 to day 60.

Conclusion

ICCs in the rat bladder show rapid proliferation since birth

and the distribution density becomes stable on day 45. The excitability of bladder is decreased gradually along with the distribution density increase of the ICCs

and then tends to be stable.

关键词

膀胱ICC增殖Ki67c-kit膀胱兴奋性

Keywords

bladder ICCproliferationKi67c-kitbladder excitability

references

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Van der Cruyssen K, De Wachter S, Van Hal G, et al. The voiding pattern in healthy pre-and term infants and toddlers: a literature review[J]. Eur J Pediatr, 2015, 174(9): 1129-1142.DOI: 10.1007/s00431-015-2578-5

王凯, 邓建平, 宋波, 等.膀胱ICC细胞的研究现状和展望[J]. 现代生物医学进展, 2012, 36(12): 7175-7178.

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