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小鼠膀胱内压力与尿道外括约肌肌电图同步测定方法的建立与验证
Development and validation of simultaneous recording of cystometrography and external urethral sphincter electromyography in mice
- 第三军医大学学报 2017年39卷第2期 页码：179-184
- 作者机构：
200433 上海, 第二军医大学长海医院泌尿外科
- 作者简介：
孙颖浩, 电话: (021) 31161719, E-mail: sunyh@medmail.com.cn Sun Yinghao, Tel: (021) 31161719, E-mail: sunyh@medmail.com.cn
- 基金信息：国家自然科学基金青年科学基金(81500579)
DOI：10.16016/j.1000-5404.201608088
中图分类号： R-334;R334;R337.5纸质出版日期：2017-1-30，
收稿日期：2016-8-18，
录用日期：2016-9-17
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宋奇翔, 叶宸, 谭海颂, 等. 小鼠膀胱内压力与尿道外括约肌肌电图同步测定方法的建立与验证[J]. 第三军医大学学报, 2017,39(2):179-184.
Qixiang Song, Chen Ye, Haisong Tan, et al. Development and validation of simultaneous recording of cystometrography and external urethral sphincter electromyography in mice[J]. J Third Mil Med Univ, 2017,39(2):179-184.
宋奇翔, 叶宸, 谭海颂, 等. 小鼠膀胱内压力与尿道外括约肌肌电图同步测定方法的建立与验证[J]. 第三军医大学学报, 2017,39(2):179-184. DOI： 10.16016/j.1000-5404.201608088.
Qixiang Song, Chen Ye, Haisong Tan, et al. Development and validation of simultaneous recording of cystometrography and external urethral sphincter electromyography in mice[J]. J Third Mil Med Univ, 2017,39(2):179-184. DOI： 10.16016/j.1000-5404.201608088.
摘要
目的
2
建立小鼠膀胱内压力(cystometrography
CMG)与尿道外括约肌肌电图(external urethral sphincter electromyography
EUS EMG)同步测定的方法，并验证其可靠性。
方法
2
将12只年龄、体质量相仿的雌性小鼠麻醉后予以耻骨上膀胱造漏并植入导管，然后进行CMG和漏尿点压(leak point pressure
LPP)测定与同步EUS EMG记录。完毕后离断双侧阴部神经，再次重复CMG、LPP及EUS EMG测定，以检测EUS EMG信号的准确性。神经损伤前后计量资料之间的比较采用配对
t
检验。
结果
2
在未损伤阴部神经时，EUS EMG在LPP处的振幅和频率 较基础膀胱压时的振幅和频率 有明显的增加(
P
＜0.001)。定量分析排尿期所出现的“EUS EMG间断性扑动”信号发现：振幅与频率分别为 (70.4±15.2)μV和(393.8±118.9)Hz，单次扑动的持续时间：(85.60±6.21)ms，两个扑动信号之间的间隔时间：(222.0±44.2) ms。而双侧阴部神经离断后，EUS EMG信号几乎完全不能被检测到，说明肌电图信号的真实性。另外，LPP、顶点膀胱压与排尿时的最大膀胱压在离断双侧阴部神经后出现了显著的降低，分别为 。
结论
2
成功建立并验证了CMG与EUS EMG同步测定在小鼠模型上的可行性。
Abstract
Objective
2
To establish and validate simultaneous recording of cystometrography (CMG) and external urethral sphincter electromyography (EUS EMG) in mice.
Methods
2
Twelve female
age-matched C57BL/6 mice were anesthetized
and the pudendal canal was located bilaterally
followed by implantation of a polyethylene catheter into the bladder for simultaneous recording of CMG
leak point pressure (LPP) and EUS EMG. After bilateral pudendal nerve transection (PNT)
the LPP and EUS EMG recording was repeated on the same mice. Statistical comparisons of quantitative data before and after PNT were made using a paired
t
-test with
P
＜0.05.
Results
2
When the pudendal nerve was intact
EUS EMG amplitude and frequency were increased significantly in response to LPP testing (from 38.9±8.4 μV to 111.7±19.4 μV and from 37.6±9.5 Hz to 142.6±15.0 Hz
respectively
P
＜0.001). Quantitative analysis of EUS EMG bursting activity during voiding was characterized with intrabursting amplitude of 70.4±15.2 μV
intrabursting firing rate of 393.8±118.9 Hz
interbursting interval of 222.0± 44.2 ms and bursting duration of 85.60±6.21 ms. Bilateral PNT eliminated EMG activity
which verified the recording quality. Besides
LPP
peak bladder pressure and maximum bladder pressure during voiding were also significantly reduced after PNT (16.4±1.2
vs
7.4±0.7 cmH
2
O
P
＜0.001; 26.7±1.7
vs
15.9±1.1 cmH
2
O
P
＜0.001; 35.5±2.0
vs
22.8±1.1 cmH
2
O
P
＜0.001
respectively).
Conclusion
2
Simultaneous recording of CMG and EUS EMG in mice is feasible
so it can be used as functional outcome in the investigation of lower urinary tract symptoms.
关键词
下尿路症状阴部神经动物模型尿动力学
Keywords
lower urinary tract symptomspudendal nerveanimal modelurodynamics
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