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网络筛选乳腺癌相关miRNAs及miR-106a-5p对乳腺癌细胞侵袭迁移能力的影响
Screening of key miRNAs related with breast cancer by bioinformatics network and effect of miR-106a-5p on invasion and migration in breast cancer cells
- 第三军医大学学报 2017年39卷第2期 页码：130-136
- 作者机构：
400037 重庆, 第三军医大学新桥医院肿瘤科
- 作者简介：
孙建国, E-mail:sunjg09@aliyun.com Sun Jianguo, E-mail:sunjg09@aliyun.com
- 基金信息：国家自然科学基金面上项目(81272910)
DOI：10.16016/j.1000-5404.201608007
中图分类号： R394-33;R394.3;R737.9纸质出版日期：2017-1-30，
收稿日期：2016-8-1，
录用日期：2016-9-30
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夏丽琴, 冯忠明, 陈旭, 等. 网络筛选乳腺癌相关miRNAs及miR-106a-5p对乳腺癌细胞侵袭迁移能力的影响[J]. 第三军医大学学报, 2017,39(2):130-136.
Liqin Xia, Zhongming Feng, Xu Chen, et al. Screening of key miRNAs related with breast cancer by bioinformatics network and effect of miR-106a-5p on invasion and migration in breast cancer cells[J]. J Third Mil Med Univ, 2017,39(2):130-136.
夏丽琴, 冯忠明, 陈旭, 等. 网络筛选乳腺癌相关miRNAs及miR-106a-5p对乳腺癌细胞侵袭迁移能力的影响[J]. 第三军医大学学报, 2017,39(2):130-136. DOI： 10.16016/j.1000-5404.201608007.
Liqin Xia, Zhongming Feng, Xu Chen, et al. Screening of key miRNAs related with breast cancer by bioinformatics network and effect of miR-106a-5p on invasion and migration in breast cancer cells[J]. J Third Mil Med Univ, 2017,39(2):130-136. DOI： 10.16016/j.1000-5404.201608007.
摘要
目的
2
网络筛选乳腺癌关键微小RNAs(microRNAs，miRNAs)，并探讨miR-106a-5p对乳腺癌细胞侵袭及迁移能力的影响。
方法
2
通过TargetScan和miRanda等工具，构建miRNAs与靶基因以及显著靶向性功能(gene ontology，GO)的调控网络，筛选对乳腺癌有关键调控作用的miRNAs；利用miR-106a-5p agomir及miR-106a-5p antagomir转染MCF-7、T47D乳腺癌细胞株，建立乳腺癌细胞中miR-106a-5p的过表达及敲低体系，Transwell侵袭实验、Transwell迁移实验、划痕实验检测miR-106a-5p对乳腺癌细胞的侵袭、迁移能力的影响。
结果
2
在miRNAs-gene-network和miRNAs-GO-network两个网络中调控维度最高的miRNAs基本一致，其中miR-106a-5p是调控维度最高的miRNAs之一；过表达miR-106a-5p显著抑制乳腺癌细胞的侵袭能力和迁移能力(
P
＜0.05)，敲低miR-106a-5p则显著增加乳腺癌细胞的侵袭能力和迁移能力(
P
＜0.05)。
结论
2
miR-106a-5p是乳腺癌miRNAs调控网络中的关键miRNAs之一，具有抑制乳腺癌细胞的侵袭及迁移能力的生物学功能。
Abstract
Objective
2
To screen key miRNAs related with breast cancer and explore the modulating effect of miR-106a-5p on the invasion and migration of breast cancer cells.
Methods
2
TargetScan and miRanda softwares were employed to predict the targets of miRNA array in breast cancer cells. The miRNAs-gene-network and miRNAs-GO-network were established based on gene ontology (GO) analysis. The overexpression and knockdown of miR-106a-5p were established by transfection of miR-106a-5p agomir and miR-106a-5p antagomir into MCF-7 and T47D breast cancer cells
respectively. The invasion and migration of breast cancer cells were tested by Transwell invasion assay
Transwell migration assay and scratch assay.
Results
2
MiR-106a-5p was one of miRNAs with the highest regulating degree in both miRNAs-gene-network and miRNAs-GO-network. The expression of miR-106a-5p in breast cancer cells was significantly lower than that in normal mammary cells (
P
＜0.05). The overexpression of miR-106a-5p inhibited the invasion and migration of MCF-7 and T47D breast cancer cells (
P
＜0.05). On the contrary
the knockdown of miR-106a-5p promoted the invasion and migration of breast cancer cells (
P
＜0.05).
Conclusion
2
miR-106a-5p is a key miRNA in the regulation network of breast cancer miRNAs
and can inhibit the invasion and migration of breast cancer cells.
关键词
miR-106a-5p生物信息学乳腺癌细胞侵袭迁移
Keywords
miR-106a-5pbioinformatics analysisbreast cancer cellsinvasionmigration
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