SOX15基因对Caco2结肠癌细胞增殖、凋亡及迁移侵袭的影响

王双; 陈欣; 姜政

doi:10.16016/j.1000-5404.201607139

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SOX15基因对Caco2结肠癌细胞增殖、凋亡及迁移侵袭的影响

基础医学 | 更新时间：2020-08-05

- SOX15基因对Caco2结肠癌细胞增殖、凋亡及迁移侵袭的影响
- Effect of SOX15 gene on proliferation, apoptosis, migration and invasion of colorectal cancer Caco2 cells
- 第三军医大学学报 2017年39卷第2期页码：137-142
- 作者机构：
  
  400016 重庆, 重庆医科大学附属第一医院消化内科
- 作者简介：
  
  姜政, E-mail:jiangz1753@163.com Jiang Zheng, E-mail:jiangz1753@163.com
- 基金信息：
- DOI：10.16016/j.1000-5404.201607139
  中图分类号： R394.3;R730.23;R735.35
- 纸质出版日期：2017-1-30，
  
  收稿日期：2016-7-21，
  
  录用日期：2016-11-2
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王双, 陈欣, 姜政. SOX15基因对Caco2结肠癌细胞增殖、凋亡及迁移侵袭的影响[J]. 第三军医大学学报, 2017,39(2):137-142.

Shuang Wang, Xin Chen, Zheng Jiang. Effect of SOX15 gene on proliferation, apoptosis, migration and invasion of colorectal cancer Caco2 cells[J]. J Third Mil Med Univ, 2017,39(2):137-142.
王双, 陈欣, 姜政. SOX15基因对Caco2结肠癌细胞增殖、凋亡及迁移侵袭的影响[J]. 第三军医大学学报, 2017,39(2):137-142. DOI： 10.16016/j.1000-5404.201607139.

Shuang Wang, Xin Chen, Zheng Jiang. Effect of SOX15 gene on proliferation, apoptosis, migration and invasion of colorectal cancer Caco2 cells[J]. J Third Mil Med Univ, 2017,39(2):137-142. DOI： 10.16016/j.1000-5404.201607139.

摘要

目的

检测SOX15在结肠癌细胞株中表达水平以及对细胞增殖、凋亡、迁移侵袭的影响。

方法

应用RT-PCR检测SOX15在Caco2、SW480、HT29、HCT116结肠癌细胞以及正常结肠组织中的表达水平。通过脂质体Lipofectamine 2000将重组质粒pEGFP-N1-SOX15及空质粒pEGFP-N1转染进Caco2细胞，应用RT-PCR及Western blot分别检测SOX15在Caco2细胞中的mRNA以及蛋白表达水平；CCK-8检测SOX15对Caco2细胞活力的影响，克隆形成实验观察细胞增殖变化，Transwell检测SOX15对细胞迁移及侵袭能力的调控，流式细胞仪分析细胞凋亡率。

结果

SOX15在Caco2、SW480、HT29、HCT116结肠癌细胞中mRNA及蛋白质表达明显低于其在正常组织中的表达水平(

＜0.01)。pEGFP-N1-SOX15 转染组细胞mRNA(1.23±0.10)和蛋白(1.13±0.08)表达显著高于pEGFP-N1对照组及空白对照组；与空白对照组及pEGFP-N1对照组相比，pEGFP-N1-SOX15 转染组可明显抑制细胞增殖及迁移侵袭能力(

＜0.01)，并诱导细胞发生凋亡(

＜0.01)。

结论

SOX15在结肠癌细胞中低表达，过表达SOX15可明显抑制结肠癌细胞Caco2的增殖及迁移、侵袭能力，并促进细胞凋亡。

Abstract

Objective

To detect the expression level of SOX15 gene in colorectal cancer cells and its effect on cell proliferation

apoptosis

migration and invasion.

Methods

The expression of SOX15 gene in colorectal cancer cell lines Caco2

SW480

HCT116

and HT29 and normal colon tissues was detected by RT-PCR. The recombinant plasmid pEGFP-N1-SOX15 and empty vector pEGFP-N1 were transfected into Caco2 cells by liposome Lipofectamine 2000

and the mRNA and protein expression levels of SOX15 gene in Caco2 cells were detected by RT-PCR and Western blotting

respectively. The viability of Caco2 cells transfected with SOX15 was tested by CCK-8 kit

and the cell proliferation was observed by clone formation assay. The regulatory effect of SOX15 on the cell migration and invasion was analyzed by Transwell assay. Flow cytometry was applied to analyze the cell apoptosis.

Results

The mRNA and protein levels of SOX15 in the Caco2

SW480

HT29

and HCT116 cells were significantly lower than those in the normal tissues. The mRNA (1.23±0.10) and protein (1.13±0.08) levels of SOX15 in the pEGFP-N1-SOX15 transfected group were obviously higher than those in the pEGFP-N1 control group (0.54±0.06 and 0.19±0.03) and in the blank control group (0.51±0.03 and 0.14±0.02

＜0.01). Compared with the blank control group and the pEGFP-N1 control group

the pEGFP-N1-SOX15 transfected group was evidently inhibited in cell viability

proliferation

migration and invasion abilities (

＜0.01)

but cell apoptosis was induced (

＜0.01).

Conclusion

SOX15 gene shows low expression in colorectal cancer cells. SOX15 over-expression can noticeably inhibit the proliferation

migration and invasion abilities

and promote cell apoptosis in Caco2 colorectal cancer cells.

关键词

SOX15结肠癌转染增殖凋亡迁移侵袭

Keywords

SOX15colorectal cancertransfectionproliferationapoptosismigrationinvasion

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