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组织非特异性碱性磷酸酶与心肌梗死后心肌纤维化的相关性研究
Correlation between Akp2 and myocardial fibrosis post myocardial infarction
- 第三军医大学学报 2022年 页码：1-7
- 作者机构：
1.实验研究中心2
2.Chongqing 400016
- 作者简介：
张冬颖，E-mail：zhangdongying@hospital.cqmu.edu.cn
- 基金信息：国家自然科学基金面上项目(31800976;81970203);中国心血管健康联盟心血管健康研究院“进阶”研究基金项目(2018-CCA-AF-034;ky-2017-07-08);Corresponding author： ZHANG Dongying, E-mail：zhangdongying@hospital.cqmu.edu.cn
DOI：10.16016/j.1000-5404.1
中图分类号： R542-
收稿日期：2019-12-31，
修回日期：2020-03-09，
扫 描 看 全 文
王立友, 雷韵祎, 高磊, 等. 组织非特异性碱性磷酸酶与心肌梗死后心肌纤维化的相关性研究[J/OL]. 第三军医大学学报, 2022,1-7.
Correlation between Akp2 and myocardial fibrosis post myocardial infarction[J/OL]. Journal of Third Military Medical University, 2022,1-7.
王立友, 雷韵祎, 高磊, 等. 组织非特异性碱性磷酸酶与心肌梗死后心肌纤维化的相关性研究[J/OL]. 第三军医大学学报, 2022,1-7. DOI： 10.16016/j.1000-5404.1.
Correlation between Akp2 and myocardial fibrosis post myocardial infarction[J/OL]. Journal of Third Military Medical University, 2022,1-7. DOI： 10.16016/j.1000-5404.1.
摘要
目的 探讨组织非特异性碱性磷酸酶（alkaline phosphatase，Akp2）与心肌梗死（myocardial infraction，MI）后心肌纤维化的关系。方法 收集2019年3—11月本院收治的46例急性心肌梗死患者为MI患者组，同期41例冠脉造影检查阴性患者为对照组，Akp2试剂盒测定血清Akp2活性，酶联免疫法测定血清Ⅰ型前胶原羧基端肽（procollagen type I C-peptide，PICP） 和Ⅲ型前胶原氨基端肽（procollagen III N-terminal peptide，PIIINP）的含量。收集5例陈旧性心肌梗死患者的心肌样本，天狼星红染色检测心脏纤维化水平，免疫组化方法检测Akp2表达情况。10只8周龄C57BL/6J雄性小鼠随机分为假手术组和心肌梗死组，每组5只，心梗术后2周取心脏标本，Masson染色检测其胶原面积，免疫组化方法检测Akp2表达情况。结果 与对照组比较，MI患者组血清Akp2活性［ （8.49±3.30）金氏单位/100 mL］显著增加（
P
<
0.01），血清PICP和PIIINP浓度均显著增加（
P
<
0.01）；血清Akp2活性与PICP和PIIINP水平呈正相关（
r
=0.38，
P
<
0.01；
r
=0.40，
P
<
0.01）。与MI患者心肌梗死远端比较，梗死边界区胶原沉积和Akp2表达均显著上调（
P
<
0.01）。与假手术组比较，心肌梗死组小鼠心脏胶原沉积显著增多（
P
<
0.01），Akp2表达增加（
P<
0.01），且Akp2的表达与纤维分布区域一致。结论 Akp2与心肌梗死后心肌纤维化病理生理过程相关。
Abstract
Objective
2
To investigate the relationship between alkaline phosphatase （Akp2） and myocardial fibrosis post myocardial infarction（MI）.
Methods
2
Forty-six patients with acute MI were enrolled as the study group and forty-one patients with negative findings in coronary angiograms during the same period were collected as the control group. The serum Akp2 activity was detected by alkaline phosphatase kit. The concentration of PICP and PIIINP were detected by enzyme-linked immunosorbent assay. Myocardial tissues were collected from five patients who died of myocardial infarction. Collagen fiber area of heart was detected by Sirius red staining and the expression of Akp2 was detected by immunohistochemistry. Ten 8-week-old male C57BL/6J mice were randomly divided into sham group and MI group with five mice in each group. Mice were sacrificed two weeks post MI，Collagen fiber area of heart was detected by Masson staining and the expression of Akp2 was detected by immunohistochemistry.
Results
2
Compared with the control group， the serum Akp2 activity was increased in patients with myocardial infarction （
P<0.01
）， the concentration of serum PICP and PIIINP in patients with myocardial infarction increased significantly （
P<0.01， P<0.01
）. Moreover， the serum Akp2 activity was significantly correlated with the concentration of serum PICP and PIIINP（r=0.38，
P<0.01
，r=0.40，
P<0.01
）.There was more collagen deposition in the infarct border area of remote myocardial infarction than in the distal infarct （
P<0.01
）
.
The expression of Akp2 was increased in patients with myocardial infarction （
P<0.01
）. Compared with the sham group， the expression of Akp2 and Collagen fiber area in MI group increased significantly （
P<0.01， P<0.01
）. The expression of Akp2 was consistent with the distribution of fibroscar in MI group.
Conclusion
2
Akp2 is related to the pathophysiological process of myocardial fibrosis after myocardial infarction.
关键词
组织非特异性碱性磷酸酶心肌梗死Ⅰ型前胶原羧基端肽Ⅲ型前胶原氨基端肽
Keywords
Akp2myocardial infarctionprocollagen type I C-peptideprocollagen III N-terminal peptide
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