OBJECTIVE: To probe into the improvement of Wenyang Tongluo method combined with biofeedback on functional anorectal pain and its effects on pain mediators

stress response and serum endothelin（ET）expression. METHODS: A total of 86 patients with functional anorectal pain admitted into the department of proctology of the hospital from Aug. 2019 to Jan. 2021 were extracted to be divided into the control group and the treatment group by random number table method

with 43 cases in each group. The control group received biofeedback therapy

while the treatment group was given Wenyang Tongluo method based on biofeedback therapy. And 10 d was a course of treatment

patients in both groups were treated for 3 courses in a row. The efficacy was calculated after the course of treatment. The visual analogue scale（VAS） score

pelvic floor electromyography parameters serum substance P（SP）

prostaglandin E2（PGE2）

bradykinin（BK）

ET-1 before and after treatment were compared between two groups. RESULTS: The total effective rate of the treatment group was 95.35%（41/43）

significantly higher than 81.40%（35/43） of the control group

the difference was statistically significant（P<0.05）. After treatment

the VAS scores of two groups were significantly lower than those before treatment

and the treatment group was significantly lower than the control group

with statistically significant differences（P<0.05）. After treatment

there was no significant difference in pelvic floor electromyography signal between two groups before and after treatment（P>0.05）. After treatment

the pelvic floor electromyography signal in the rapid contraction stage

post-rest stage in two groups were significantly lower than those before treatment

endurance contraction stage and the pelvic floor electromyography signal in the continuous contraction stage was significantly higher than that before treatment

the differences were statistically significant（P<0.05）. After treatment

the pelvic floor electromyography signal of the treatment group was significantly lower than that of the control group in the rapid contraction stage

post-rest stage

and endurance contraction stage

the pelvic floor electromyography signal of the treatment group was significantly higher than that of the control group in the continuous contraction stage

the differences were statistically significant（P<0.05）. After treatment

the serum SP

PGE2

BK

ET-1 of two groups were significantly lower than those before treatment

and the serum SP

PGE2

BK

ET-1 of the treatment group were significantly lower than those of the control group

the differences were statistically significant（P<0.05）. CONCLUSIONS: The efficacy of Wenyang Tongluo method combined with biofeedback on functional anorectal pain is significant

which can effectively reduce the pain level

the secretion of pain mediators

the stress response of the body

and the serum ET content.