OBJECTIVE: To probe into the effects of autologous platelet-rich plasma（PRP） combined with granulocyte colony-stimulating factor（G-CSF） for intrauterine perfusion on endometrial receptivity（ER） and clinical outcome in patients with repeated implantation failure（RIF）. METHODS: Totally 76 patients with RIF admitted into this hospital from Jan. 2020 to Jul. 2022 were selected and divided into PRP+G-CSF perfusion group（38 cases） and control group（no perfusion

38 cases） according to whether or not intrauterine perfusion was performed before frozen-thawed embryo transfer. The embryo implantation rates

chemical pregnancy rates

clinical pregnancy rates

early miscarriage rates and ectopic pregnancy rates of both groups were recorded

the thickness

morphology

blood flow type and hemodynamic indexes [uterine artery pulsatility index（PI）

resistance index（RI）] of endometrium were compared between two groups on the day of embryo transfer

and the incidence of adverse events of uterine perfusion in the PRP+G-CSF group was recorded. RESULTS: After treatment

the embryo implantation rate

chemical pregnancy rate and clinical pregnancy rate of the PRP+G-CSF group were respectively 46.88%（30/38）

60.53%（23/38） and 60.53%（23/38）

which were higher than those of the control group [26.56%（17/38）

31.58%（12/38） and 31.58%（12/38）]

with statistically significant differences（P<0.05）. The early abortion rates were 4.35%（1/23） and 16.67%（2/12） in the two groups

with no statistically significant difference（P>0.05）. No ectopic pregnancy occurred in both groups

and there were no adverse events of intrauterine perfusion in the PRP+G-CSF group. On the day of embryo transfer

the endometrial thickness of patients in the PRP+G-CSF group was（10.20±2.02） mm

which was thicker than that of the control group [（9.15±1.51） mm]

with statistically significant difference（P<0.05）; the percentage of endometrial blood flow type GⅡ or above and the percentage of C-type endometrium were respectively 86.84%（33/38） and 89.47%（34/38）

which were higher than those in the control group [65.79%（25/38） and 71.05%（27/38）]

with statistically significant differences（P<0.05）. On the day of embryo transfer

the uterine artery PI and RI levels of patients in the PRP+G-CSF group were 2.35±0.53 and 0.83±0.08

which were lower than those of the control group（2.69±0.49 and 0.87±0.09）

with statistically significant differences（P<0.05）. CONCLUSIONS: PRP combined with G-CSF intrauterine perfusion can improve endometrial thickness and blood perfusion in patients with RIF

thus improve their ER and pregnancy outcomes.