OBJECTIVE: To probe into the potential active components and possible mechanism of Mailuo Shutong pills in the treatment of hemorrhoids. METHODS: Chemical constituents of Mailuo Shutong pills and the related targets of hemorrhoids were obtained by Traditional Chinese medicine systems pharmacology database and analysis platform

DisGeNET

GeneCards and other databases. The intersection targets were imported into STRING database for analysis

and the analysis results were imported into Cytoscape software to construct protein-protein interaction（PPI） network diagram

Mailuo Shutong pills chemical components-target network diagram and drug-component-target-pathway network. The key targets were analyzed by gene ontology（GO） enrichment analysis and Kyoto Encyclopedia of Gene and Genome（KEGG） pathway enrichment analysis by R language. AutoDockTools software was used to verify the molecular docking between the active components of Mailuo Shutong pills and the corresponding key targets. RESULTS: A total of 215 compounds

310 related targets

and 148 compound and disease intersection targets were screened. Key targets including protein kinase B（Akt） 1

tumor protein p53（TP53）

interleukin（IL） 6

epidermal growth factor receptor（EGFR）

vascular endothelial growth factor A（VEGFA）

ESR1

HIF1 A and tumor necrosis factor（TNF） were identified. Based on the analysis of PPI network

there were 161 KEGG pathways and 3 041 GO functional entries were obtained. In the GO functional items

there were 2 706 biological process related items

140 cellular components

and 195 molecular function related items. The results of molecular docking showed that quercetin

formononetin

luteolin

wogonin

kaempferol and other compounds had good binding activity with Akt1

TNF

TP53

IL-6

EGFR

VEGFA and ESR1. CONCLUSIONS: Mailuo Shutong pills in the treatment of hemorrhoids is based on the mechanism of multi-components and multi-targets. This study provides theoretical basis for further verification of related mechanisms.