绿原酸通过Bax/Bcl-2/Caspase-3信号通路对喉癌Hep-2细胞增殖、凋亡的影响

张立坤; 邵东风; 王东海; 李扬

doi:10.14009/j.issn.1672-2124.2022.10.012

您当前的位置：

首页 >

文章列表页 >

绿原酸通过Bax/Bcl-2/Caspase-3信号通路对喉癌Hep-2细胞增殖、凋亡的影响

论著 | 更新时间：2023-04-16

- 绿原酸通过Bax/Bcl-2/Caspase-3信号通路对喉癌Hep-2细胞增殖、凋亡的影响
- Effects of Chlorogenic Acid on Proliferation and Apoptosis of Laryngeal Cancer Hep-2 Cells Through Bax/Bcl-2/Caspase-3 Signaling Pathway
- 中国医院用药评价与分析 2022年22卷第10期页码：1206-1210
- 作者机构：
  
  1. 唐山市协和医院耳鼻咽喉头颈外科
  2. 华北理工大学附属医院呼吸与危重症医学科
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2022.10.012
  中图分类号： R739.65
- 纸质出版日期：2022，
扫描看全文
张立坤, 邵东风, 王东海, 等. 绿原酸通过Bax/Bcl-2/Caspase-3信号通路对喉癌Hep-2细胞增殖、凋亡的影响[J]. 中国医院用药评价与分析, 2022,22(10):1206-1210.

ZHANG Likun, SHAO Dongfeng, WANG Donghai, et al. Effects of Chlorogenic Acid on Proliferation and Apoptosis of Laryngeal Cancer Hep-2 Cells Through Bax/Bcl-2/Caspase-3 Signaling Pathway[J]. 2022,22(10):1206-1210.
张立坤, 邵东风, 王东海, 等. 绿原酸通过Bax/Bcl-2/Caspase-3信号通路对喉癌Hep-2细胞增殖、凋亡的影响[J]. 中国医院用药评价与分析, 2022,22(10):1206-1210. DOI： 10.14009/j.issn.1672-2124.2022.10.012.

ZHANG Likun, SHAO Dongfeng, WANG Donghai, et al. Effects of Chlorogenic Acid on Proliferation and Apoptosis of Laryngeal Cancer Hep-2 Cells Through Bax/Bcl-2/Caspase-3 Signaling Pathway[J]. 2022,22(10):1206-1210. DOI： 10.14009/j.issn.1672-2124.2022.10.012.

摘要

目的：探讨绿原酸(CGA)对喉癌Hep-2细胞增殖、凋亡及对Bcl-2相关X蛋白(Bax)/B淋巴细胞瘤2(Bcl-2)/胱天蛋白酶-3(Caspase-3)信号通路的影响。方法：分别用含有0、250、500、750和1 000μmol/L的CGA培养液处理喉癌Hep-2细胞24、48 h

寻找出最适宜CGA的浓度和处理时间；将喉癌Hep-2细胞分为对照组(正常培养的喉癌Hep-2细胞)、CGA低浓度组(750μmol/L CGA培养液)、CGA高浓度组(1 000μmol/L CGA培养液)和CGA高浓度+通路抑制剂组(1 000μmol/L CGA培养液+50μmol/L Bax/Bcl-2/Caspase-3通路抑制剂Bax inhibitor peptide V5)。采用细胞计数试剂盒-8(CCK-8)法测定喉癌Hep-2细胞增殖抑制率，通过流式细胞仪检测喉癌Hep-2细胞凋亡率，采用蛋白质印迹法检测喉癌Hep-2细胞中增殖相关蛋白(ki-67)、凋亡相关蛋白(p53)及Bax/Bcl-2/Caspase-3信号通路相关蛋白的表达情况。结果：与对照组相比，CGA低、高浓度组喉癌Hep-2细胞增殖抑制率、细胞凋亡率，p53、Bax和Caspase-3蛋白表达水平显著升高，ki-67、Bcl-2蛋白表达水平显著降低，差异均有统计学意义(P<0.05);且CGA高浓度组喉癌Hep-2细胞增殖抑制率、细胞凋亡率，p53、Bax和Caspase-3蛋白表达水平高于CGA低浓度组，ki-67、Bcl-2蛋白表达水平低于CGA低浓度组，差异均有统计学意义(P<0.05)。与CGA高浓度组相比，CGA高浓度+通路抑制剂组喉癌Hep-2细胞增殖抑制率、细胞凋亡率，p53、Bax和Caspase-3蛋白表达水平显著降低，ki-67、Bcl-2蛋白表达水平显著升高，差异均有统计学意义(P<0.05)。结论：CGA可显著抑制喉癌Hep-2细胞的增殖，并促进其凋亡，可能与Bax/Bcl-2/Caspase-3信号通路激活有关。

Abstract

OBJECTIVE: To probe into the effects of chlorogenic acid（CGA） on proliferation and apoptosis of laryngeal cancer Hep-2 cells through Bcl-2-associated X protein（Bax）/B cell lymphoma-2（Bcl-2）/cysteinyl aspartate specific proteinase-3（Caspase-3）. METHODS: Laryngeal cancer Hep-2 cells were treated with CGA culture medium of 0

250

500

750 and 1 000 μmol/L for 24 and 48 h respectively to find out the most suitable concentration and treatment time of CGA. Laryngeal cancer Hep-2 cells were divided into the control group（normally cultured laryngeal cancer Hep-2 cells）

CGA low concentration group（750 μmol/L CGA medium）

CGA high concentration group（1 000 μmol/L CGA medium）

and CGA high concentration+pathway inhibitor group（1 000 μmol/L CGA medium+50 μmol/L Bax/Bcl-2/Caspase-3 pathway inhibitor Bax inhibitor peptide V5）. Cell counting kit-8（CCK-8） method was used to determine the proliferation inhibition rate of laryngeal cancer Hep-2 cells in each group

flow cytometry was used to detect the apoptosis rate of laryngeal cancer Hep-2 cells in each group

Western blotting was used to determine the expression of proliferation related protein（ki-67）

apoptosis related protein（p53） and Bax/Bcl-2/Caspase-3 signaling pathway related proteins in laryngeal cancer Hep-2 cells. RESULTS: Compared with the control group

the proliferation inhibition rate

apoptosis rate

p53

Bax and Caspase-3 protein expression levels of laryngeal cancer Hep-2 cells in CGA low and high concentration group were significantly higher

and the ki-67 and Bcl-2 protein expression levels were significantly lower

with statistically significant differences（P<0.05）; and the proliferation inhibition rate

apoptosis rate

p53

Bax and Caspase-3 protein expression levels of laryngeal cancer Hep-2 cells in CGA high concentration group were higher than those in CGA low concentration group

the of ki-67 and Bcl-2 protein expression levels were lower than those in CGA low concentration group

with statistically significant differences（P<0.05）. Compared with the CGA high concentration group

the proliferation inhibition rate

apoptosis rate

p53

Bax and Caspase-3 protein expression levels of laryngeal cancer Hep-2 cells in the CGA high concentration+pathway inhibitor group were significantly lower

and the ki-67 and Bcl-2 protein expression levels were significantly higher

with statistically significant differences（P<0.05）. CONCLUSIONS: CGA can significantly inhibit the proliferation of laryngeal cancer Hep-2 cells and promote the apoptosis

which may be related to the activation of Bax/Bcl-2/Caspase-3 signaling pathway.

关键词

绿原酸喉癌Hep-2细胞Bcl-2相关X蛋白B淋巴细胞瘤2胱天蛋白酶-3

Keywords

Chlorogenic acidLaryngeal cancer Hep-2 cellsBcl-2-associated X proteinB cell lymphoma-2Cysteinyl aspartate specific proteinase-3

references

查看原文

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

暂无数据