OBJECTIVE: To probe into the effects of quercitrin on alleviation and improvement of model mice with anaphylactic rhinitis（AR） and the related mechanisms. METHODS: The mice were randomly divided into the control group

the model group（ovalbumin） and the treatment group（ovalbumin + quercitrin）

with 10 mice in each group. The AR model of the model group was established by “ovalbumin injection and nasal mucosa stimulation method”

namely

the mice were injected intraperitoneally with ovalbumin 0.3 mg

Al（OH）

3

30 mg and 0.9% sodium chloride solution 1 mL after initial immunization

and the mice were stimulated with 4% ooalbumin solution（20 μL/mouse） on each nostril from 15 th to 21 st day. Mice in the control group were intraperitoneally injected with Al（OH）

3

and then stimulated with 0.9% sodium chloride solution from 15 th to 21 st day. On the basis of the model group

mice in the treatment group were given quercitrin 50 mg/kg intragastric administration at the same time of nostril stimulation from the 15 th to 21 st day. The number of nasal friction and sneezing were recorded after the last ovalbumin stimulation. Hematoxylin-eosin staining and periodic acid-Schiff staining were performed on the nasal septum of mice in each group. Levels of inflammatory immune factors tumor necrosis factor α（TNF-α）

interleukin（IL）-4

IL-10 and IL-17 in nasal lavage fluid of mice were detected. Diff-Quik staining was performed on cells in nasal lavage fluid. Immunofluorescence staining of olfactory marker protein（OMP） in nasal mucosa epithelial cells of mice in each group was performed. RESULTS: The average number of sneezing and nasal friction in the treatment group was significantly less than that in the model group

with statistically significant difference（P

<

0.01）. Compared with the control group

the nasal septum tissue of mice in the model group showed obvious goblet cell proliferation and active mucus secretion. Compared with the model group

goblet cell proliferation and mucus secretion in nasal septum tissue of mice in the treatment group decreased. Compared with the model group

the levels of TNF-α

IL-4

IL-10 and IL-17 in nasal lavage fluid of mice in the treatment group decreased significantly

with statistically significant difference（P

<

0.01）. Compared with the control group

the total number of cells and differentiated cells（including epithelial cells

eosinophils

neutrophils

lymphocytes and macrophages） in the nasal lavage fluid of the mice in the model group increased significantly; compared with the model group

the number of cells in the nasal lavage fluid of the mice in the treatment group decreased significantly. The expression of OMP in olfactory epithelial cells decreased significantly in the model group. However

the reduction of OMP in olfactory epithelial cells of mice in the treatment group was reversed to a certain extent and the expression of OMP increased. CONCLUSIONS: Quercitrin can improve AR symptoms by reducing inflammatory immune response and increasing OMP expression in nasal mucosal epithelial cells.