OBJECTIVE: To study the repair of oxidative stress injury and regulation of nuclear factor κB（NF-κB） pathway in rats with α-naphthalene isothiocyanate（ANIT）-induced intrahepatic cholestasis by Dahuang granule prescription. METHODS: A total of 60 rats were randomly divided into the control group

model group

Dahuang granule prescription group and positive control group. The rat model of intrahepatic cholestasis was established by intragastric administration of ANIT. Rats in the Dahuang granule prescription group were intragastrically administered with Dahuang granule prescription 4 g/kg

rats in the positive control group were intragastrically administered with ursodeoxycholic acid 100 mg/kg

and rats in the control group and model group received the same amount of 0.9% sodium chloride solution by gavage

qd

for 14 d. The levels of alanine aminotransferase（ALT）

aspartate aminotransferase（AST）

alkaline phosphatase（ALP）

glutyltransferase（GGT）

total bilirubin（TBIL） and direct bilirubin（DBIL） in serum

the levels of superoxide dismutase（SOD）

malondialdehyde（MDA） and glutathione（GSH） in liver tissue were determined. Liver histopathology was performed by Hematoxylin-Eosin staining

and protein levels of NF-κB p65 and p-NF-κB p65 were detected by western blotting. RESULTS: Compared with the model group

the levels of ALT

AST

ALP

GGT

TBIL and DBIL in serum

the levels of MDA and GSH

NF-κB p65 and p-NF-κB p65 in the liver tissue of the Dahuang granule prescription group decreased significantly

the SOD level increased significantly

the differences were statistically significant（P<0.05）. The liver histopathology of rats was significantly improved. CONCLUSIONS: Dahuang granule prescription can significantly repair the liver function and oxidative stress injury of liver tissue

and improve the bile metabolism level

which may be related to the regulation of NF-κB pathway.