OBJECTIVE: To explore the network pharmacological mechanism of Guizhi Fuling pill in the treatment of endometriosis. METHODS: The main active ingredients of Guizhi Fuling pill（Guizhi

Fuling

Chishao

Taoren

Mudanpi） were selected by database and literature mining

and the targets were collected. Meanwhile

endometriosis targets were obtained by disease database. The common targets of Guizhi Fuling pill and endometriosis were analyzed

and the common targets and the neighbor nodes were selected to construct the “ingredient target-disease target” core network. Key targets were screened for Gene Ontology（GO） functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes（KEGG） pathway enrichment analysis

to analyze the possible biological process and signal pathway of Guizhi Fuling pill in the treatment of EMT. RESULTS: Totally 1 600 endometriosis targets were obtained. After screening

51 active ingredients and 823 targets were identified in Guizhi Fuling pill

there were 190 targets for diseases and drugs. Results of GO functional enrichment analysis showed that Guizhi Fuling pill might regulate modulating cannabinoid receptor 1（CB1）

cannabinoid receptor 2（CB2）

erythrocyte sedimentation rate（ESR）

progesterone receptor（PGR） and ALOX-5 and other targets

which were closely related to protein

DNA and enzyme binding

and were involved in many biological processes such as apoptosis

proliferation and positive regulation of transcription. KEGG pathway enrichment analysis showed that the above targets were related to the regulation of mitogen-activated protein kinase（MAPK） and other signaling pathways. CONCLUSIONS: Through network pharmacology

it is found that Guizhi Fuling pill could regulate the MAPK signaling pathway by regulating the targets of CB1

CB2

ESR

PGR and ALOX-5

and intervene in the pathological processes of endometriosis such as proliferation

anti-apoptosis

adhesion

invasion and angiogenesis

and exert the mechanism of “blood stasis and phlegm-dispelling”.