OBJECTIVE: To probe into the effects of Danshensu（Dss） on steroid receptor coactivator/focal adhesion kinase（Src/FAK） signaling pathway and epithelial-mesenchymal transition（EMT） in bladder cancer 5637 cells. METHODS: Bladder cancer 5637 cells were treated with different concentrations of Dss（0 μg/mL

40 μg/mL

60 μg/mL and 80 μg/mL） for 24 h

and were named Dss-0 group

Dss-40 group

Dss-60 group and Dss-80 group

respectively. Real time fluorescence quantitative polymerase chain reaction was used to detect the mRNA expression level of EMT marker genes [E-cadherin

N-cadherin

Vimentin messenger RNA（mRNA）] in bladder cancer 5637 cells. Western blot was used to detect the expression level of related proteins in the Src/FAK signaling pathway in bladder cancer 5637 cells. Cell count kit 8（CCK-8） was used to detect the proliferation of bladder cancer 5637 cells. The scratch test was used to detect bladder cancer 5637 cells migration. RESULTS: In Dss-0 group

Dss-40 group

Dss-60 group and Dss-80 group

the expression level of E-cadherin mRNA in bladder cancer 5637 cells increased in sequence

N-cadherin mRNA

Vimentin mRNA

phosphorylated Src（p-Src）/Src protein

phosphorylated FAK（p-FAK）/FAK protein expression levels

absorbance（OD） and migration rate decreased in a concentration dependent manner（P<0.05）. CONCLUSIONS: Dss may inhibit the EMT process of bladder cancer 5637 cells by inhibiting the Src/FAK signaling pathway

thereby inhibiting the proliferation and migration of bladder cancer 5637 cells.