复方苦参注射液对多发性骨髓瘤RPMI8226细胞增殖和凋亡的影响

刘蕊; 范宁建; 王英曼; 宋洁; 张岩

doi:10.14009/j.issn.1672-2124.2022.03.011

您当前的位置：

首页 >

文章列表页 >

复方苦参注射液对多发性骨髓瘤RPMI8226细胞增殖和凋亡的影响

论著 | 更新时间：2022-10-08

- 复方苦参注射液对多发性骨髓瘤RPMI8226细胞增殖和凋亡的影响
- Effects of Compound Kushen Injection on Proliferation and Apoptosis of RPMI8226 Cells in Multiple Myeloma
- 中国医院用药评价与分析 2022年22卷第3期页码：300-303
- 作者机构：
  
  1. 华北理工大学附属医院肿瘤放化疗科
  2. 唐山市第二医院脊柱三科
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2022.03.011
  中图分类号： R285
- 纸质出版日期：2022，
扫描看全文
刘蕊, 范宁建, 王英曼, 等. 复方苦参注射液对多发性骨髓瘤RPMI8226细胞增殖和凋亡的影响[J]. 中国医院用药评价与分析, 2022,22(3):300-303.

LIU Rui, FAN Ningjian, WANG Yingman, et al. Effects of Compound Kushen Injection on Proliferation and Apoptosis of RPMI8226 Cells in Multiple Myeloma[J]. 2022,22(3):300-303.
刘蕊, 范宁建, 王英曼, 等. 复方苦参注射液对多发性骨髓瘤RPMI8226细胞增殖和凋亡的影响[J]. 中国医院用药评价与分析, 2022,22(3):300-303. DOI： 10.14009/j.issn.1672-2124.2022.03.011.

LIU Rui, FAN Ningjian, WANG Yingman, et al. Effects of Compound Kushen Injection on Proliferation and Apoptosis of RPMI8226 Cells in Multiple Myeloma[J]. 2022,22(3):300-303. DOI： 10.14009/j.issn.1672-2124.2022.03.011.

摘要

目的：探讨复方苦参注射液对多发性骨髓瘤RPMI8226细胞增殖和凋亡的影响。方法：体外培养多发性骨髓瘤RPMI8226细胞系，采用四甲基偶氮唑盐法检测不同浓度复方苦参注射液对细胞增殖的影响，采用流式细胞仪检测细胞周期和凋亡，采用酶联免疫吸附法检测白细胞介素6（IL-6）的表达水平。结果：0.5、1、2、5和10 mg/mL的复方苦参注射液均有抑制骨髓瘤RPMI8226细胞增殖的作用，且呈时间和剂量依赖性。5、10 mg/mL的复方苦参注射液可显著促进细胞凋亡，并阻滞细胞周期于G

期。复方苦参注射液治疗后，细胞IL-6表达水平明显下调。结论：复方苦参注射液能抑制骨髓瘤细胞的增殖，诱导凋亡，其机制可能与抑制IL-6的表达有关。

Abstract

OBJECTIVE: To probe into the effects of compound Kushen injection on proliferation and apoptosis of RPMI8226 cells in multiple myeloma. METHODS: RPMI8226 cells in multiple myeloma was subcultured in vitro. The effects of different concentrations of compound Kushen injection on cell proliferation were detected by Methyl thiazolyl tetrazolium

and cell cycle and apoptosis were detected by flow cytometry. The expression of interleukin-6（IL-6） was detected by enzyme-linked immunosorbent assay. RESULTS: Compound Kushen injection of 0.5 mg/mL

1 mg/mL

2 mg/mL

5 mg/mL and 10 mg/mL could inhibit the proliferation of RPMI8226 cells in multiple myeloma in a time and dose dependent manner. Compound Kushen injection of 5 mg/mL and 10 mg/mL could significantly promote cell apoptosis and block cell cycle in G

phase. The expression of IL-6 decreased significantly after the treatment of compound Kushen injection. CONCLUSIONS: Compound Kushen injection can inhibit the proliferation and induce the apoptosis of RPMI8226 cells

and its mechanism may be related to the inhibition of IL-6 expression.

关键词

多发性骨髓瘤复方苦参注射增殖白细胞介素6

Keywords

Multiple myelomaCompound Kushen injectionProliferationInterleukin-6

references

查看原文

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

地佐辛对成骨细胞MC3T3-E1增殖、分化及对Wnt/β-catenin信号通路的影响

澳洲茄碱通过MAPK/ERK信号通路调控肝癌细胞增殖、迁移和侵袭的研究

FESS术后口服香菊胶囊联合布地奈德喷鼻治疗慢性鼻窦炎伴有鼻息肉的疗效及对患者血清IL-2、IL-6和CRP水平的影响

淫羊藿苷对肾癌OS-RC-2细胞增殖、迁移、凋亡及PTEN/AKT通路的影响

核桃青皮提取物对乳腺癌MCF-7细胞增殖、凋亡及EGFR/MAPK信号通路的影响