OBJECTIVE: To probe into the potential mechanism of Chaishao Liujunzi decoction in prevention and treatment of chemotherapy-induced nausea and vomiting（ CINV） based on liver-soothing and spleen-strengthening method. METHODS: The pharmacological database and analysis platform of traditional Chinese medicine system was used to search the chemical components and potential targets of drug composition of Chaishao Liujunzi decoction

including Bupleuri Radix

Paeoniae Radix Alba

Codonopsis Radix

Poria

Atractylodis Macrocephalae Rhizoma

Citri Reticulatae Pericarpium

Pinelliae Rhizoma

and Glycyrrhizae Radix et Rhizoma

oral bioavailability ≥30% and druglikeness ≥0. 18 were set as the screening conditions of chemical composition; the GeneCards database was used to retrieve disease targets of CINV; Cytoscape 3. 6. 0 software was used to draw the network of “Chaishao Liujunzi decoction-chemical components-target-CINV”; STRING 11. 0 online software was used to construct a protein-protein interaction network and to mine the core targets; David Bioinformatics Resources database was used to conduct gene ontology（ GO） functional enrichment analysis and Kyoto Encyclopedia of Gene and Genome（ KEGG） pathway enrichment analysis on protein in the network of potential targets of active ingredients in the formula. RESULTS: The GO functional enrichment analysis indicated that Chaishao Liujunzi decoction had extensively participated in biological processes including body metabolism and inflammation. The KEGG pathway enrichment analysis indicated that all the signaling pathways of Chaishao Liujunzi decoction were related to inflammation

the disease pathways were mainly closely related to virus infection and various malignant tumor. CONCLUSIONS: This study predicts the possible signaling pathways of prevention and treatment of CINV of Chaishao Liujunzi decoction from the perspective of network pharmacology

a representative formula of liver-soothing and spleen-strengthening method

which laid a certain foundation for the subsequent mechanism research.