OBJECTIVE: To establish a method of ultra-high performance liquid chromatography-tandem mass spectrometry for the content determination of toosendanin in Jinhong tablets. METHODS: Chromatographic column of Phenomenex Kinetex XB C

18

（ 100 mm × 2. 1 mm

2. 6 μm） was adopted

water and acetonitrile were used as mobile phases in a gradient elution at a flow rate of 0. 5 ml/min

with the ion source was ESI

-

which was detected by multiple reaction detection scan. RESULTS: The linearity was good in the range of 0. 01-200 μg/L（ r = 0. 997 9）

with the limit of quantification was 0. 01 μg/L. This method had good repeatability and precision

with the recoveries of 96. 9%-105. 8% and the RSD of 1. 88%-3. 21% at the three levels of low

medium and high. Resuolts of stability test showed that the test solution was stable within 48 h at room temperature（ 25 ℃） ． CONCLUSIONS: This method has a large linear range

good linearity

high precision and short analysis time

which can be used for the quality control of toosendanin in Jinhong tablets.